351D

X-RAY CRYSTAL STRUCTURES OF THE HEXAMER DCACGCG: CRYSTALS GROWN IN THE PRESENCE OF RUTHENIUM (II) HEXAMMINE CHLORIDE

351D の概要

• エントリーDOI: 10.2210/pdb351d/pdb
• 分子名称: DNA (5'-D(*CP*AP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*TP*G)-3') (3 entities in total)
• 機能のキーワード: z-dna, double helix, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3619.42

構造登録者

Karthe, P.,Gautham, N. (登録日: 1997-08-30, 公開日: 1997-09-30, 最終更新日: 2024-02-21)

主引用文献

Karthe, P.,Gautham, N.
Structure of d(CACGCG).d(CGCGTG) in crystals grown in the presence of ruthenium III hexammine chloride.
Acta Crystallogr.,Sect.D, 54:501-509, 1998

PubMed Abstract: Hexammine ions are strong inducers of the transition from the B-form to the left-handed Z-form in DNA. Here the structure of d(CACGCG). d(CGCGTG) obtained from crystals grown from a drop containing [Ru(NH3)6]Cl3 is reported. The structure is clearly characterized as Z-DNA. When compared with the structure of d(CACGCG).d(CGCGTG)/MgCl2 and that of d(CGCGCG)2, subtle differences are seen, most noticeably in the water structure. Since stable well diffracting crystals grow easily in the presence of [Ru(NH3)6]Cl3 and since this ion is not visible in the electron density it is concluded that the ion plays a non-specific role in stabilizing Z-DNA.

PubMed: 9761846
DOI: 10.1107/S0907444997013954

実験手法

X-RAY DIFFRACTION (1.64 Å)