348D

X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION

348D の概要

• エントリーDOI: 10.2210/pdb348d/pdb
• 分子名称: DNA (5'-D(*GP*AP*CP*CP*GP*CP*GP*GP*TP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna, double helix, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3045.99

構造登録者

Luo, M.,Finley, J.B. (登録日: 1997-08-29, 公開日: 1997-10-09, 最終更新日: 2024-02-21)

主引用文献

Finley, J.B.,Luo, M.
X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC).
Nucleic Acids Res., 26:5719-5727, 1998

PubMed Abstract: The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.

PubMed: 9838004
DOI: 10.1093/nar/26.24.5719

実験手法

X-RAY DIFFRACTION (1.7 Å)