33BF

Single particle reconstruction of Rhodospirillum rubrum encapsulated ferritin in encapsulin nano compartment

「9S13」から置き換えられました

33BF の概要

• エントリーDOI: 10.2210/pdb33bf/pdb
• EMDBエントリー: 59321
• 分子名称: Encapsulated ferritin-like protein, FE (III) ION (2 entities in total)
• 機能のキーワード: encapsulin, nanocompartment, encapsulated ferritin, structural protein, oxidoreductase
• 由来する生物種: Rhodospirillum rubrum
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 152675.65

構造登録者

Marles-Wright, J.,McIver, Z.,Ross, J.,McCorvie, T.,Basle, A. (登録日: 2026-08-05, 公開日: 2026-09-02)

主引用文献

McIver, Z.,He, D.,Ross, J.,Cozzaglio, M.,Piergentili, C.,Dornau, A.,Sumpner, N.,Brady, F.,Bialik, K.,McCorvie, T.,Sissi, C.,Basle, A.,Clarke, D.J.,Marles-Wright, J.
Single particle cryo-EM analysis of the Rhodospirillum rubrum encapsulin nanocompartment shows variable loading of encapsulated ferritin cargo proteins and differences in the fivefold pore.
J.Struct.Biol., 218:108357-108357, 2026

PubMed Abstract: Encapsulins are self-assembling protein nanocompartments found in bacteria and archaea that encapsulate cargo enzymes to protect the cell from their toxic reaction products or intermediates. Developments in cryo-electron microscopy (cryo-EM) data processing strategies have enabled encapsulins and their cargo proteins to be investigated together in greater detail. In this study, we present the single particle cryo-EM structure of the Rhodospirillum rubrum encapsulin in both the presence and absence of its partner encapsulated ferritin (EncFtn). Single particle icosahedral reconstructions of empty and loaded encapsulins revealed a higher degree of conformational flexibility at the five-fold pore in the cargo loaded encapsulin. We applied a new non-point group averaging workflow to analyze the encapsulated ferritins within the encapsulin nanocompartment, to produce the first fully refined in situ atomic model of the EncFtn at 2.8 Å resolution. Masked 2D classification and particle subtraction demonstrate that cargo loading is heterogeneous in this recombinant complex, with the encapsulin able to house up to five of the decameric EncFtn complexes. Our data provides new insights into the dynamics and cargo arrangement in encapsulins and demonstrates an adaptable workflow for high resolution reconstruction of encapsulin cargoes.

PubMed: 42603627
DOI: 10.1016/j.jsb.2026.108357

実験手法

ELECTRON MICROSCOPY (2.79 Å)