31YO

Horse spleen Apoferritin 0.45 MGy variable temperature dose series, 100 K

31YO の概要

• エントリーDOI: 10.2210/pdb31yo/pdb
• 分子名称: Ferritin light chain, CADMIUM ION (3 entities in total)
• 機能のキーワード: metal storage, dose series, temperature series, metal binding protein
• 由来する生物種: Equus caballus (horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20209.66

構造登録者

Southworth-Davies, R.J.,Garman, E.F. (登録日: 2026-06-29, 公開日: 2026-08-26, 最終更新日: 2026-09-30)

主引用文献

Southworth-Davies, R.J.,Murray, J.W.,Carmichael, I.,Rudino-Pinera, E.,Weik, M.,Garman, E.F.
Separating the effects of temperature and absorbed X-ray dose on unit-cell volume.
Acta Crystallogr D Struct Biol, 2026

PubMed Abstract: The behaviour of the unit-cell volume of crystals of the iron-storage molecule ferritin, both in the apo and the holo form, and of influenza A virus subtype N9 neuraminidase at 100 K and over a controlled cryo-temperature series was investigated. The purpose of this study was to separate out the effects of dose and temperature on the protein by assessing the changes in the unit-cell volume. Over the wide range of X-ray doses examined in this work, the irreversible effect of dose on the unit-cell volume could be distinguished from the reversible (below 160 K) temperature-induced effects. Specific structural damage effects were not reversible.

PubMed: 42741972
DOI: 10.1107/S2059798326008612

実験手法

X-RAY DIFFRACTION (2.15 Å)