300D
CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MN(II)-SOAKED
Summary for 300D
| Entry DOI | 10.2210/pdb300d/pdb |
| Descriptor | RNA HAMMERHEAD RIBOZYME, MANGANESE (II) ION (3 entities in total) |
| Functional Keywords | rna hammerhead ribozyme, catalytic rna, loop, ribozyme |
| Total number of polymer chains | 2 |
| Total formula weight | 13519.61 |
| Authors | Scott, W.G.,Murray, J.B.,Arnold, J.R.P.,Stoddard, B.L.,Klug, A. (deposition date: 1996-12-14, release date: 1997-01-24, Last modification date: 2024-02-21) |
| Primary citation | Scott, W.G.,Murray, J.B.,Arnold, J.R.,Stoddard, B.L.,Klug, A. Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme. Science, 274:2065-2069, 1996 Cited by PubMed Abstract: The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state. PubMed: 8953035DOI: 10.1126/science.274.5295.2065 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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