2ZYW

crystal structure of mouse cytosolic sulfotransferase mSULT1D1 complex with PAP and p-nitrophenol, obtained by two-step soaking method

2ZYW の概要

• エントリーDOI: 10.2210/pdb2zyw/pdb
• 関連するPDBエントリー: 2ZPT 2ZVP 2ZVQ 2ZYT 2ZYU 2ZYV
• 分子名称: Tyrosine-ester sulfotransferase, ADENOSINE-3'-5'-DIPHOSPHATE, P-NITROPHENOL, ... (5 entities in total)
• 機能のキーワード: sult1d1, sult, sulfotransferase, pap, p-nitrophenol, sulfonation, transferase
• 由来する生物種: Mus musculus (mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36109.99

構造登録者

Teramoto, T.,Sakakibara, Y.,Liu, M.-C.,Suiko, M.,Kimura, M.,Kakuta, Y. (登録日: 2009-01-29, 公開日: 2009-04-21, 最終更新日: 2023-11-01)

主引用文献

Teramoto, T.,Sakakibara, Y.,Liu, M.-C.,Suiko, M.,Kimura, M.,Kakuta, Y.
Snapshot of a Michaelis complex in a sulfuryl transfer reaction: Crystal structure of a mouse sulfotransferase, mSULT1D1, complexed with donor substrate and accepter substrate
Biochem.Biophys.Res.Commun., 383:83-87, 2009

PubMed Abstract: We report the crystal structure of mouse sulfotransferase, mSULT1D1, complexed with donor substrate 3'-phosphoadenosine 5'-phosphosulfate and accepter substrate p-nitrophenol. The structure is the first report of the native Michaelis complex of sulfotransferase. In the structure, three proposed catalytic residues (Lys48, Lys106, and His108) were in proper positions for engaging in the sulfuryl transfer reaction. The data strongly support that the sulfuryl transfer reaction proceeds through an S(N)2-like in-line displacement mechanism.

PubMed: 19344693
DOI: 10.1016/j.bbrc.2009.03.146

実験手法

X-RAY DIFFRACTION (1.8 Å)