2ZXR

Crystal structure of RecJ in complex with Mg2+ from Thermus thermophilus HB8

2ZXR の概要

• エントリーDOI: 10.2210/pdb2zxr/pdb
• 関連するPDBエントリー: 2ZXO 2ZXP
• 分子名称: Single-stranded DNA specific exonuclease RecJ, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: nuclease, single-stranded dna, dna repair, hydrolase, exonuclease
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72984.65

構造登録者

Wakamatsu, T.,Kitamura, Y.,Nakagawa, N.,Masui, R.,Kuramitsu, S. (登録日: 2009-01-05, 公開日: 2010-01-05, 最終更新日: 2023-11-01)

主引用文献

Wakamatsu, T.,Kitamura, Y.,Kotera, Y.,Nakagawa, N.,Kuramitsu, S.,Masui, R.
Structure of RecJ exonuclease defines its specificity for single-stranded DNA
J.Biol.Chem., 285:9762-9769, 2010

PubMed Abstract: RecJ is a single-stranded DNA (ssDNA)-specific 5'-3' exonuclease that plays an important role in DNA repair and recombination. To elucidate how RecJ achieves its high specificity for ssDNA, we determined the entire structures of RecJ both in a ligand-free form and in a complex with Mn(2+) or Mg(2+) by x-ray crystallography. The entire RecJ consists of four domains that form a molecule with an O-like structure. One of two newly identified domains had structural similarities to an oligonucleotide/oligosaccharide-binding (OB) fold. The OB fold domain alone could bind to DNA, indicating that this domain is a novel member of the OB fold superfamily. The truncated RecJ containing only the core domain exhibited much lower affinity for the ssDNA substrate compared with intact RecJ. These results support the hypothesis that these structural features allow specific binding of RecJ to ssDNA. In addition, the structure of the RecJ-Mn(2+) complex suggests that the hydrolysis reaction catalyzed by RecJ proceeds through a two-metal ion mechanism.

PubMed: 20129927
DOI: 10.1074/jbc.M109.096487

実験手法

X-RAY DIFFRACTION (2.15 Å)