2ZUG

Crystal structure of WSSV ICP11

2ZUG の概要

• エントリーDOI: 10.2210/pdb2zug/pdb
• 関連するPDBエントリー: 2GJ2
• 分子名称: ORF115 (WSSV285) (Wsv230) (2 entities in total)
• 機能のキーワード: dna mimic protein, dimer, white spot syndrome virus, viral protein
• 由来する生物種: Shrimp white spot syndrome virus (WSSV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20954.23

構造登録者

Wang, A.H.-J.,Wang, H.-C.,Ko, T.-P.,Lo, C.-F. (登録日: 2008-10-17, 公開日: 2008-12-09, 最終更新日: 2024-10-30)

主引用文献

Wang, H.-C.,Wang, H.-C.,Ko, T.-P.,Lee, Y.-M.,Leu, J.-H.,Ho, C.-H.,Huang, W.-P.,Lo, C.-F.,Wang, A.H.-J.
White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly
Proc.Natl.Acad.Sci.USA, 105:20758-20763, 2008

PubMed Abstract: White spot syndrome virus (WSSV) is a large ( approximately 300 kbp), double-stranded DNA eukaryotic virus that has caused serious disease in crustaceans worldwide. ICP11 is the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance in WSSV infection; but until now, its function has remained obscure. We show here that ICP11 acts as a DNA mimic. In crystal, ICP11 formed a polymer of dimers with 2 rows of negatively charged spots that approximated the duplex arrangement of the phosphate groups in DNA. Functionally, ICP11 prevented DNA from binding to histone proteins H2A, H2B, H3, and H2A.x, and in hemocytes from WSSV-infected shrimp, ICP11 colocalized with histone H3 and activated-H2A.x. These observations together suggest that ICP11 might interfere with nucleosome assembly and prevent H2A.x from fulfilling its critical function of repairing DNA double strand breaks. Therefore, ICP11 possesses a functionality that is unique among the handful of presently known DNA mimic proteins.

PubMed: 19095797
DOI: 10.1073/pnas.0811233106

実験手法

X-RAY DIFFRACTION (2.72 Å)