2ZRT

Crystal structure of Zn2+-bound form of des3-23ALG-2

2ZRT の概要

• エントリーDOI: 10.2210/pdb2zrt/pdb
• 関連するPDBエントリー: 2ZN9 2ZND 2ZNE 2ZRS
• 分子名称: Programmed cell death protein 6, ZINC ION (2 entities in total)
• 機能のキーワード: penta-ef-hand protein, calcium-binding protein, apoptosis, calcium, endoplasmic reticulum, membrane, nucleus, polymorphism
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus membrane; Peripheral membrane protein: O75340
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 160868.64

構造登録者

Suzuki, H.,Kawasaki, M.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M. (登録日: 2008-09-01, 公開日: 2008-11-04, 最終更新日: 2023-11-01)

主引用文献

Suzuki, H.,Kawasaki, M.,Kakiuchi, T.,Shibata, H.,Wakatsuki, S.,Maki, M.
Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2
ACTA CRYSTALLOGR.,SECT.F, 64:974-977, 2008

PubMed Abstract: ALG-2 (apoptosis-linked gene 2) is an apoptosis-linked calcium-binding protein with five EF-hand motifs in the C-terminal region. N-terminally truncated ALG-2 (des3-23ALG-2) was crystallized by the vapour-diffusion method in buffer consisting of either 50 mM MES pH 6.5, 12.5%(v/v) 2-propanol and 150 mM calcium acetate or 100 mM MES pH 6.0, 15%(v/v) ethanol and 200 mM zinc acetate. Crystals of the Ca(2+)-bound form belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 54.8, b = 154.4, c = 237.7 A, alpha = beta = gamma = 90 degrees , and diffracted to 3.1 A resolution. Crystals of the Zn(2+)-bound form belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.8, b = 147.5, c = 230.7 A, alpha = beta = gamma = 90 degrees , and diffracted to 3.3 A resolution. The structures of the Ca(2+)-bound form and the Zn(2+)-bound form were solved by the molecular-replacement method. Although both crystals contained eight ALG-2 molecules per asymmetric unit, the metal-ion locations and octameric arrangements were found to be significantly different.

PubMed: 18997320
DOI: 10.1107/S1744309108030297

実験手法

X-RAY DIFFRACTION (3.3 Å)