2ZRQ

Crystal structure of S324A-subtilisin

2ZRQ の概要

• エントリーDOI: 10.2210/pdb2zrq/pdb
• 関連するPDBエントリー: 2Z2X
• 分子名称: Tk-subtilisin, CALCIUM ION (3 entities in total)
• 機能のキーワード: subtilisin, thermococcus kodakaraensis, calcium, hydrolase, protease, secreted, serine protease, zymogen
• 由来する生物種: Pyrococcus kodakaraensis (Thermococcus kodakaraensis)
• 細胞内の位置: Secreted: P58502
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34178.11

構造登録者

Tanaka, S.,Takeuchi, Y.,Matsumura, H.,Koga, Y.,Takano, K.,Kanaya, S. (登録日: 2008-08-28, 公開日: 2009-03-03, 最終更新日: 2024-11-13)

主引用文献

Tanaka, S.,Takeuchi, Y.,Matsumura, H.,Koga, Y.,Takano, K.,Kanaya, S.
Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding
Febs Lett., 582:3875-3878, 2008

PubMed Abstract: Tk-subtilisin (a subtilisin homologue from Thermococcus kodakaraensis) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide. To analyze the folding mechanism of Tk-subtilisin, the crystal structure of the active site mutant of Tk-subtilisin (S324A-subtilisin*), which was refolded in the presence of Ca2+ and absence of Tk-propeptide, was determined at 2.16A resolution. This structure is essentially the same as that of Tk-subtilisin matured from Pro-Tk-subtilisin. S324A-subtilisin* was refolded with a rate constant of 0.17 and 1.8min(-1) at 30 degrees C in the absence and presence of Tk-propeptide, respectively, indicating that Tk-subtilisin does not require Tk-propeptide for folding but requires it for acceleration of folding.

PubMed: 18951896
DOI: 10.1016/j.febslet.2008.10.025

実験手法

X-RAY DIFFRACTION (2.16 Å)