2ZQO

Crystal structure of the earthworm R-type lectin C-half in complex with GalNAc

「2AO3」から置き換えられました

2ZQO の概要

• エントリーDOI: 10.2210/pdb2zqo/pdb
• 関連するPDBエントリー: 2DRY 2DRZ 2DS0 2ZQN
• 分子名称: 29-kDa galactose-binding lectin, 2-acetamido-2-deoxy-beta-D-galactopyranose, CADMIUM ION, ... (6 entities in total)
• 機能のキーワード: earthworm lumbricus terrestris, hemagglutinin, r-type lectin, beta-trefoil fold, sugar complex, lectin, sugar binding protein
• 由来する生物種: Lumbricus terrestris (Common earthworm)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30585.98

構造登録者

Suzuki, R.,Kuno, A.,Hasegawa, T.,Hirabayashi, J.,Kasai, K.,Momma, M.,Fujimoto, Z. (登録日: 2008-08-13, 公開日: 2008-09-02, 最終更新日: 2023-11-01)

主引用文献

Suzuki, R.,Kuno, A.,Hasegawa, T.,Hirabayashi, J.,Kasai, K.,Momma, M.,Fujimoto, Z.
Sugar-complex structures of the C-half domain of the galactose-binding lectin EW29 from the earthworm Lumbricus terrestris
Acta Crystallogr.,Sect.D, 65:49-57, 2009

PubMed Abstract: R-type lectins are one of the most prominent types of lectin; they exist ubiquitously in nature and mainly bind to the galactose unit of sugar chains. The galactose-binding lectin EW29 from the earthworm Lumbricus terrestris belongs to the R-type lectin family as represented by the plant lectin ricin. It shows haemagglutination activity and is composed of a single peptide chain that includes two homologous domains: N-terminal and C-terminal domains. A truncated mutant of EW29 comprising the C-terminal domain (rC-half) has haemagglutination activity by itself. In order to clarify how rC-half recognizes ligands and shows haemagglutination activity, X-ray crystal structures of rC-half in complex with D-lactose and N-acetyl-D-galactosamine have been determined. The structure of rC-half is similar to that of the ricin B chain and consists of a beta-trefoil fold; the fold is further divided into three similar subdomains referred to as subdomains alpha, beta and gamma, which are gathered around the pseudo-threefold axis. The structures of sugar complexes demonstrated that subdomains alpha and gamma of rC-half bind terminal galactosyl and N-acetylgalactosaminyl glycans. The sugar-binding properties are common to both ligands in both subdomains and are quite similar to those of ricin B chain-lactose complexes. These results indicate that the C-terminal domain of EW29 uses these two galactose-binding sites for its function as a single-domain-type haemagglutinin.

PubMed: 19153466
DOI: 10.1107/S0907444908037451

実験手法

X-RAY DIFFRACTION (1.8 Å)