2ZP8

The Nature of the TRAP:Anti-TRAP complex

2ZP8 の概要

• エントリーDOI: 10.2210/pdb2zp8/pdb
• 関連するPDBエントリー: 2ZP9
• 分子名称: Transcription attenuation protein mtrB, Tryptophan RNA-binding attenuator protein-inhibitory protein, TRYPTOPHAN, ... (4 entities in total)
• 機能のキーワード: protein-protein complex, transcription, rna-binding, transcription regulation, rna binding protein-transcription complex, rna binding protein/transcription
• 由来する生物種: Bacillus stearothermophilus; 詳細
• 細胞内の位置: Cytoplasm (Probable): O31466
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 68172.25

構造登録者

Watanabe, M.,Heddle, J.G.,Unzai, S.,Akashi, S.,Park, S.Y.,Tame, J.R.H. (登録日: 2008-07-08, 公開日: 2009-02-03, 最終更新日: 2023-11-01)

主引用文献

Watanabe, M.,Heddle, J.G.,Kikuchi, K.,Unzai, S.,Akashi, S.,Park, S.Y.,Tame, J.R.
The nature of the TRAP-Anti-TRAP complex.
Proc.Natl.Acad.Sci.USA, 106:2176-2181, 2009

PubMed Abstract: Tryptophan biosynthesis is subject to exquisite control in species of Bacillus and has become one of the best-studied model systems in gene regulation. The protein TRAP (trp RNA-binding attenuation protein) predominantly forms a ring-shaped 11-mer, which binds cognate RNA in the presence of tryptophan to suppress expression of the trp operon. TRAP is itself regulated by the protein Anti-TRAP, which binds to TRAP and prevents RNA binding. To date, the nature of this interaction has proved elusive. Here, we describe mass spectrometry and analytical centrifugation studies of the complex, and 2 crystal structures of the TRAP-Anti-TRAP complex. These crystal structures, both refined to 3.2-A resolution, show that Anti-TRAP binds to TRAP as a trimer, sterically blocking RNA binding. Mass spectrometry shows that 11-mer TRAP may bind up to 5 AT trimers, and an artificial 12-mer TRAP may bind 6. Both forms of TRAP make the same interactions with Anti-TRAP. Crystallization of wild-type TRAP with Anti-TRAP selectively pulls the 12-mer TRAP form out of solution, so the crystal structure of wild-type TRAP-Anti-TRAP complex reflects a minor species from a mixed population.

PubMed: 19164760
DOI: 10.1073/pnas.0801032106

実験手法

X-RAY DIFFRACTION (3.2 Å)