2ZMW
Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0
Replaces: 2EJPSummary for 2ZMW
| Entry DOI | 10.2210/pdb2zmw/pdb |
| Related | 2ZMU |
| Descriptor | Fluorescent protein (2 entities in total) |
| Functional Keywords | gfp-like protein, luminescent protein, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi |
| Biological source | Fungia concinna (Mushroom coral) |
| Total number of polymer chains | 4 |
| Total formula weight | 102151.47 |
| Authors | Kikuchi, A.,Fukumura, E.,Karasawa, S.,Mizuno, H.,Miyawaki, A.,Shiro, Y.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2008-04-21, release date: 2008-10-21, Last modification date: 2024-10-16) |
| Primary citation | Kikuchi, A.,Fukumura, E.,Karasawa, S.,Mizuno, H.,Miyawaki, A.,Shiro, Y. Structural Characterization of a Thiazoline-Containing Chromophore in an Orange Fluorescent Protein, Monomeric Kusabira Orange Biochemistry, 47:11573-11580, 2008 Cited by PubMed Abstract: Monomeric Kusabira Orange (mKO) is a green fluorescent protein (GFP)-like protein that emits orange light at a peak of 559 nm. We analyzed its X-ray structure at 1.65 A and found a novel three-ring chromophore that developed autocatalytically from a Cys65-Tyr66-Glu67 tripeptide in which the side chain of Cys65 formed the third 2-hydroxy-3-thiazoline ring. As a result, the chromophore contained the CNCOH group at the 2-position of the imidazolinone moiety such that the conjugated pi-electron system of the chromophore was more extended than that of GFP but less extended than that of the Discosoma sp. red fluorescent protein (DsRed). Since a sulfur atom has potent nucleophilic character, the third 3-thiazoline ring is rapidly and completely cyclized. Furthermore, our structure reveals the presence of a pi-pi stacking interaction between His197 and the chromophore as well as a pi-cation interaction between Arg69 and the chromophore. These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO. PubMed: 18844376DOI: 10.1021/bi800727v PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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