2ZMW

Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0

「2EJP」から置き換えられました

2ZMW の概要

• エントリーDOI: 10.2210/pdb2zmw/pdb
• 関連するPDBエントリー: 2ZMU
• 分子名称: Fluorescent protein (2 entities in total)
• 機能のキーワード: gfp-like protein, luminescent protein, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Fungia concinna (Mushroom coral)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 102151.47

構造登録者

Kikuchi, A.,Fukumura, E.,Karasawa, S.,Mizuno, H.,Miyawaki, A.,Shiro, Y.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2008-04-21, 公開日: 2008-10-21, 最終更新日: 2024-10-16)

主引用文献

Kikuchi, A.,Fukumura, E.,Karasawa, S.,Mizuno, H.,Miyawaki, A.,Shiro, Y.
Structural Characterization of a Thiazoline-Containing Chromophore in an Orange Fluorescent Protein, Monomeric Kusabira Orange
Biochemistry, 47:11573-11580, 2008

PubMed Abstract: Monomeric Kusabira Orange (mKO) is a green fluorescent protein (GFP)-like protein that emits orange light at a peak of 559 nm. We analyzed its X-ray structure at 1.65 A and found a novel three-ring chromophore that developed autocatalytically from a Cys65-Tyr66-Glu67 tripeptide in which the side chain of Cys65 formed the third 2-hydroxy-3-thiazoline ring. As a result, the chromophore contained the CNCOH group at the 2-position of the imidazolinone moiety such that the conjugated pi-electron system of the chromophore was more extended than that of GFP but less extended than that of the Discosoma sp. red fluorescent protein (DsRed). Since a sulfur atom has potent nucleophilic character, the third 3-thiazoline ring is rapidly and completely cyclized. Furthermore, our structure reveals the presence of a pi-pi stacking interaction between His197 and the chromophore as well as a pi-cation interaction between Arg69 and the chromophore. These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO.

PubMed: 18844376
DOI: 10.1021/bi800727v

実験手法

X-RAY DIFFRACTION (2 Å)