2ZLD

Structure of OmpF co-crystallized with T83

2ZLD の概要

• エントリーDOI: 10.2210/pdb2zld/pdb
• 関連するPDBエントリー: 2ZFG
• 分子名称: Outer membrane protein F, Colicin-E3 (2 entities in total)
• 機能のキーワード: btub, colicin e3, ribosomal rnaase, disordered t83, tol system, ion transport, membrane, outer membrane, phage recognition, porin, transmembrane, transport, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P02931
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 75456.00

構造登録者

Cramer, W.A.,Zakharov, S.D.,Yamashita, E. (登録日: 2008-04-09, 公開日: 2008-07-29, 最終更新日: 2023-11-01)

主引用文献

Yamashita, E.,Zhalnina, M.V.,Zakharov, S.D.,Sharma, O.,Cramer, W.A.
Crystal structures of the OmpF porin: function in a colicin translocon.
Embo J., 27:2171-2180, 2008

PubMed Abstract: The OmpF porin in the Escherichia coli outer membrane (OM) is required for the cytotoxic action of group A colicins, which are proposed to insert their translocation and active domains through OmpF pores. A crystal structure was sought of OmpF with an inserted colicin segment. A 1.6 A OmpF structure, obtained from crystals formed in 1 M Mg2+, has one Mg2+ bound in the selectivity filter between Asp113 and Glu117 of loop 3. Co-crystallization of OmpF with the unfolded 83 residue glycine-rich N-terminal segment of colicin E3 (T83) that occludes OmpF ion channels yielded a 3.0 A structure with inserted T83, which was obtained without Mg2+ as was T83 binding to OmpF. The incremental electron density could be modelled as an extended poly-glycine peptide of at least seven residues. It overlapped the Mg2+ binding site obtained without T83, explaining the absence of peptide binding in the presence of Mg2+. Involvement of OmpF in colicin passage through the OM was further documented by immuno-extraction of an OM complex, the colicin translocon, consisting of colicin E3, BtuB and OmpF.

PubMed: 18636093
DOI: 10.1038/emboj.2008.137

実験手法

X-RAY DIFFRACTION (3 Å)