2ZJA

Archaeal DNA helicase Hjm complexed with AMPPCP in form 2

2ZJA の概要

• エントリーDOI: 10.2210/pdb2zja/pdb
• 関連するPDBエントリー: 2ZJ2 2ZJ5 2ZJ8
• 分子名称: Putative ski2-type helicase, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER (3 entities in total)
• 機能のキーワード: reca fold, atp-binding, helicase, hydrolase, nucleotide-binding
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 83251.61

構造登録者

Oyama, T.,Oka, H.,Fujikane, R.,Ishino, Y.,Morikawa, K. (登録日: 2008-02-29, 公開日: 2009-02-10, 最終更新日: 2023-11-01)

主引用文献

Oyama, T.,Oka, H.,Mayanagi, K.,Shirai, T.,Matoba, K.,Fujikane, R.,Ishino, Y.,Morikawa, K.
Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm
Bmc Struct.Biol., 9:2-2, 2009

PubMed Abstract: Pyrococcus furiosus Hjm (PfuHjm) is a structure-specific DNA helicase that was originally identified by in vitro screening for Holliday junction migration activity. It belongs to helicase superfamily 2, and shares homology with the human DNA polymerase Theta (PolTheta), HEL308, and Drosophila Mus308 proteins, which are involved in DNA repair. Previous biochemical and genetic analyses revealed that PfuHjm preferentially binds to fork-related Y-structured DNAs and unwinds their double-stranded regions, suggesting that this helicase is a functional counterpart of the bacterial RecQ helicase, which is essential for genome maintenance. Elucidation of the DNA unwinding and translocation mechanisms by PfuHjm will require its three-dimensional structure at atomic resolution.

PubMed: 19159486
DOI: 10.1186/1472-6807-9-2

実験手法

X-RAY DIFFRACTION (2.7 Å)