2ZHL

Crystal structure of human galectin-9 N-terminal CRD in complex with N-acetyllactosamine dimer (crystal 2)

2ZHL の概要

• エントリーDOI: 10.2210/pdb2zhl/pdb
• 関連するPDBエントリー: 2ZHK 2ZHM 2ZHN
• 分子名称: Galectin-9, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: beta sandwich, carbohydrate binding protein, galectin, sugar binding protein
• 由来する生物種: HOMO SAPIENS (human)
• 細胞内の位置: Cytoplasm (By similarity): O00182
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 68428.14

構造登録者

Nagae, M.,Nishi, N.,Murata, T.,Usui, T.,Nakamura, T.,Wakatsuki, S.,Kato, R. (登録日: 2008-02-06, 公開日: 2008-12-30, 最終更新日: 2024-03-13)

主引用文献

Nagae, M.,Nishi, N.,Murata, T.,Usui, T.,Nakamura, T.,Wakatsuki, S.,Kato, R.
Structural analysis of the recognition mechanism of poly-N-acetyllactosamine by the human galectin-9 N-terminal carbohydrate recognition domain.
Glycobiology, 19:112-117, 2009

PubMed Abstract: Galectins are a family of beta-galactoside-specific lectins bearing a conserved carbohydrate recognition domain. Interactions between galectins and poly-N-acetyllactosamine sequences are critical in a variety of biological processes. Galectin-9, a member of the galectin family, has two carbohydrate recognition domains at both the N- and C-terminal regions. Here we report the crystal structure of the human galectin-9 N-terminal carbohydrate recognition domain in complex with N-acetyllactosamine dimers and trimers. These complex structures revealed that the galectin-9 N-terminal carbohydrate recognition domain can recognize internal N-acetyllactosamine units within poly-N-acetyllactosamine chains. Based on these complex structures, we propose two putative recognition modes for poly-N-acetyllactosamine binding by galectins.

PubMed: 18977853
DOI: 10.1093/glycob/cwn121

実験手法

X-RAY DIFFRACTION (1.75 Å)