2ZH8

Complex structure of AFCCA with tRNAminiDGC

2ZH8 の概要

• エントリーDOI: 10.2210/pdb2zh8/pdb
• 関連するPDBエントリー: 2ZH1 2ZH2 2ZH3 2ZH4 2ZH5 2ZH6 2ZH7 2ZH9 2ZHA 2ZHB
• 分子名称: tRNA (34-MER), CCA-adding enzyme, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: transferase/rna, atp-binding, magnesium, metal-binding, nucleotide-binding, nucleotidyltransferase, rna repair, rna-binding, trna processing, transferase-rna complex
• 由来する生物種: Archaeoglobus fulgidus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62605.79

構造登録者

Toh, Y.,Tomita, K. (登録日: 2008-02-01, 公開日: 2008-08-05, 最終更新日: 2024-03-13)

主引用文献

Toh, Y.,Numata, T.,Watanabe, K.,Takeshita, D.,Nureki, O.,Tomita, K.
Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme
Embo J., 27:1944-1952, 2008

PubMed Abstract: CCA-adding enzyme builds the 3'-end CCA of tRNA without a nucleic acid template. The mechanism for the maintenance of fidelity during the CCA-adding reaction remains elusive. Here, we present almost a dozen complex structures of the class I CCA-adding enzyme and tRNA mini-helices (mini-D(73)N(74), mini-D(73)N(74)C(75) and mini-D(73)C(74)N(75); D(73) is a discriminator nucleotide and N is either A, G, or U). The mini-D(73)N(74) complexes adopt catalytically inactive open forms, and CTP shifts the enzymes to the active closed forms and allows N(74) to flip for CMP incorporation. In contrast, unlike the catalytically active closed form of the mini-D(73)C(74)C(75) complex, the mini-D(73)N(74)C(75) and mini-D(73)C(74)N(75) complexes adopt inactive open forms. Only the mini-D(73)C(74)U(75) accepts AMP to a similar extent as mini-D(73)C(74)C(75), and ATP shifts the enzyme to a closed, active form and allows U(75) to flip for AMP incorporation. These findings suggest that the 3'-region of RNA is proofread, after two nucleotide additions, in the closed, active form of the complex at the AMP incorporation stage. This proofreading is a prerequisite for the maintenance of fidelity for complete CCA synthesis.

PubMed: 18583961
DOI: 10.1038/emboj.2008.124

実験手法

X-RAY DIFFRACTION (2.65 Å)