2ZE0

Alpha-glucosidase GSJ

Summary for 2ZE0

• Entry DOI: 10.2210/pdb2ze0/pdb
• Descriptor: Alpha-glucosidase, CALCIUM ION (3 entities in total)
• Functional Keywords: tim barrel, glucoside hydrolase, extremophile, hydrolase
• Biological source: Geobacillus sp.
• Total number of polymer chains: 1
• Total formula weight: 65300.23

Authors

Shirai, T.,Hung, V.S.,Morinaka, K.,Kobayashi, T.,Ito, S. (deposition date: 2007-12-04, release date: 2007-12-18, Last modification date: 2023-11-01)

Primary citation

Shirai, T.,Hung, V.S.,Morinaka, K.,Kobayashi, T.,Ito, S.
Crystal structure of GH13 alpha-glucosidase GSJ from one of the deepest sea bacteria
Proteins, 73:126-133, 2008

PubMed Abstract: The crystal structure of the GH13 alpha-glucosidase (GSJ) from deep-sea bacterium Geobacillus sp. strain HTA-462 was determined to a 2.0 A resolution. Comparisons of the GSJ structure with that of other GH13 enzymes with different catalytic activities revealed that the catalytic cleft of GSJ was widely opened when compared with the homologues. The wide opening of the catalytic cleft originated from conformational changes of active site residues and disorder of the regions close to the catalytic center. This structural feature of GSJ would explain the ability of this enzyme to accept a wide variety of nonsugar molecules as acceptors in the transglycosylation reaction.

PubMed: 18398906
DOI: 10.1002/prot.22044

Experimental method

X-RAY DIFFRACTION (2 Å)