2ZE0

Alpha-glucosidase GSJ

2ZE0 の概要

• エントリーDOI: 10.2210/pdb2ze0/pdb
• 分子名称: Alpha-glucosidase, CALCIUM ION (3 entities in total)
• 機能のキーワード: tim barrel, glucoside hydrolase, extremophile, hydrolase
• 由来する生物種: Geobacillus sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 65300.23

構造登録者

Shirai, T.,Hung, V.S.,Morinaka, K.,Kobayashi, T.,Ito, S. (登録日: 2007-12-04, 公開日: 2007-12-18, 最終更新日: 2023-11-01)

主引用文献

Shirai, T.,Hung, V.S.,Morinaka, K.,Kobayashi, T.,Ito, S.
Crystal structure of GH13 alpha-glucosidase GSJ from one of the deepest sea bacteria
Proteins, 73:126-133, 2008

PubMed Abstract: The crystal structure of the GH13 alpha-glucosidase (GSJ) from deep-sea bacterium Geobacillus sp. strain HTA-462 was determined to a 2.0 A resolution. Comparisons of the GSJ structure with that of other GH13 enzymes with different catalytic activities revealed that the catalytic cleft of GSJ was widely opened when compared with the homologues. The wide opening of the catalytic cleft originated from conformational changes of active site residues and disorder of the regions close to the catalytic center. This structural feature of GSJ would explain the ability of this enzyme to accept a wide variety of nonsugar molecules as acceptors in the transglycosylation reaction.

PubMed: 18398906
DOI: 10.1002/prot.22044

実験手法

X-RAY DIFFRACTION (2 Å)