2ZBJ

Crystal structure of Dioclea rostrata lectin

2ZBJ の概要

• エントリーDOI: 10.2210/pdb2zbj/pdb
• 分子名称: Lectin alpha chain, PHOSPHATE ION, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: leguminosae, lectin, diocleinae, dioclea rostrata, calcium, manganese, metal-binding, vacuole, sugar binding protein
• 由来する生物種: Dioclea rostrata
• 細胞内の位置: Vacuole, aleurone grain: P58908
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25703.18

構造登録者

de Oliveira, T.M.,Delatorre, P.,da Rocha, B.A.M.,de Sousa, E.P.,Nascimento, K.S.,Bezerra, G.A.,Moura, T.R.,Benevides, R.G.,Bezerra, E.H.S.,Moreno, F.B.M.B.,Freire, V.N.,de Azevedo Jr., W.F.,Cavada, B.S. (登録日: 2007-10-22, 公開日: 2008-08-19, 最終更新日: 2023-11-01)

主引用文献

de Oliveira, T.M.,Delatorre, P.,da Rocha, B.A.M.,de Souza, E.P.,Nascimento, K.S.,Bezerra, G.A.,Moura, T.R.,Benevides, R.G.,Bezerra, E.H.S.,Moreno, F.B.M.B.,Freire, V.N.,de Azevedo Jr., W.F.,Cavada, B.S.
Crystal structure of Dioclea rostrata lectin: Insights into understanding the pH-dependent dimer-tetramer equilibrium and the structural basis for carbohydrate recognition in Diocleinae lectins
J.Struct.Biol., 164:177-182, 2008

PubMed Abstract: The legume lectins from the subtribe Diocleinae, often referred to as concanavalin A-like lectins, are a typical example of highly similar proteins that show distinct biological activities. The pH-dependent oligomerization that some of these lectins undergo and the relative position of amino acids within the carbohydrate-binding site are factors that have been reported to contribute to these differences in the activities of Diocleinae lectins. In the present work, we determined the amino acid sequence and the crystal structure of the lectin of Dioclea rostrata seeds (DRL), with the aim of investigating the structural bases of the different behavior displayed by this lectin in comparison to other Diocleinae lectins and determining the reason for the distinct pH-dependent dimer-tetramer equilibrium. In addition, we discovered a novel multimeric arrangement for this lectin.

PubMed: 18682294
DOI: 10.1016/j.jsb.2008.05.012

実験手法

X-RAY DIFFRACTION (2.05 Å)