2Z9X

Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-L-alanine

Summary for 2Z9X

• Entry DOI: 10.2210/pdb2z9x/pdb
• Related: 2Z9U 2Z9V 2Z9W
• Descriptor: Aspartate aminotransferase, SULFATE ION, CHLORIDE ION, ... (7 entities in total)
• Functional Keywords: aminotransferase, pyridoxamine, pyruvate, alanine, transferase
• Biological source: Mesorhizobium loti
• Total number of polymer chains: 2
• Total formula weight: 85279.88

Authors

Yoshikane, Y.,Yokochi, N.,Yamasaki, M.,Mizutani, K.,Ohnishi, K.,Mikami, B.,Hayashi, H.,Yagi, T. (deposition date: 2007-09-26, release date: 2007-11-06, Last modification date: 2023-11-01)

Primary citation

Yoshikane, Y.,Yokochi, N.,Yamasaki, M.,Mizutani, K.,Ohnishi, K.,Mikami, B.,Hayashi, H.,Yagi, T.
Crystal structure of pyridoxamine-pyruvate aminotransferase from Mesorhizobium loti MAFF303099
J.Biol.Chem., 283:1120-1127, 2008

PubMed Abstract: Pyridoxamine-pyruvate aminotransferase (PPAT; EC 2.6.1.30) is a pyridoxal 5'-phosphate-independent aminotransferase and catalyzes reversible transamination between pyridoxamine and pyruvate to form pyridoxal and L-alanine. The crystal structure of PPAT from Mesorhizobium loti has been solved in space group P4(3)2(1)2 and was refined to an R factor of 15.6% (R(free) = 20.6%) at 2.0 A resolution. In addition, the structures of PPAT in complexes with pyridoxamine, pyridoxal, and pyridoxyl-L-alanine have been refined to R factors of 15.6, 15.4, and 14.5% (R(free) = 18.6, 18.1, and 18.4%) at 1.7, 1.7, and 2.0 A resolution, respectively. PPAT is a homotetramer and each subunit is composed of a large N-terminal domain, consisting of seven beta-sheets and eight alpha-helices, and a smaller C-terminal domain, consisting of three beta-sheets and four alpha-helices. The substrate pyridoxal is bound through an aldimine linkage to Lys-197 in the active site. The alpha-carboxylate group of the substrate amino/keto acid is hydrogen-bonded to Arg-336 and Arg-345. The structures revealed that the bulky side chain of Glu-68 interfered with the binding of the phosphate moiety of pyridoxal 5'-phosphate and made PPAT specific to pyridoxal. The reaction mechanism of the enzyme is discussed based on the structures and kinetics results.

PubMed: 17989071
DOI: 10.1074/jbc.M708061200

Experimental method

X-RAY DIFFRACTION (1.94 Å)