2Z8U

Methanococcus jannaschii TBP

2Z8U の概要

• エントリーDOI: 10.2210/pdb2z8u/pdb
• 分子名称: TATA-box-binding protein (2 entities in total)
• 機能のキーワード: transcription, dna-binding protein, transcription factor, transcription regulation
• 由来する生物種: Methanococcus jannaschii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83757.16

構造登録者

Adachi, N.,Senda, T.,Horikoshi, M. (登録日: 2007-09-10, 公開日: 2008-08-26, 最終更新日: 2023-11-01)

主引用文献

Adachi, N.,Senda, M.,Natsume, R.,Senda, T.,Horikoshi, M.
Crystal structure of Methanococcus jannaschii TATA box-binding protein.
Genes Cells, 13:1127-1140, 2008

PubMed Abstract: As the archaeal transcription system consists of a eukaryotic-type transcription apparatus and bacterial-type regulatory transcription factors, analyses of the molecular interface between the transcription apparatus and regulatory transcription factors are critical to reveal the evolutionary change of the transcription system. TATA box-binding protein (TBP), the central components of the transcription apparatus are classified into three groups: eukaryotic, archaeal-I and archaeal-II TBPs. Thus, comparative functional analysis of these three groups of TBP is important for the study of the evolution of the transcription system. Here, we present the first crystal structure of an archaeal-II TBP from Methanococcus jannaschii. The highly conserved and group-specific conserved surfaces of TBP bind to DNA and TFIIB/TFB, respectively. The phylogenetic trees of TBP and TFIIB/TFB revealed that they evolved in a coupled manner. The diversified surface of TBP is negatively charged in the archaeal-II TBP, which is completely different from the case of eukaryotic and archaeal-I TBPs, which are positively charged and biphasic, respectively. This difference is responsible for the diversification of the regulatory functions of TBP during evolution.

PubMed: 19090808
DOI: 10.1111/j.1365-2443.2008.01233.x

実験手法

X-RAY DIFFRACTION (1.9 Å)