2Z4T

Crystal Structure of Vibrionaceae Photobacterium sp. JT-ISH-224 2,6-sialyltransferase in a Ternary Complex with Donor Product CMP and Accepter Substrate Lactose

2Z4T の概要

• エントリーDOI: 10.2210/pdb2z4t/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900008
• 分子名称: Beta-galactoside alpha-2,6-sialyltransferase, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, CYTIDINE-5'-MONOPHOSPHATE, ... (7 entities in total)
• 機能のキーワード: gt-b fold, immunoglobulin-like beta-sandwich fold, glycosyltransferase, transferase
• 由来する生物種: Photobacterium sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59960.59

構造登録者

Kakuta, Y.,Okino, N.,Kajiwara, H.,Ichikawa, M.,Takakura, Y.,Ito, M.,Yamamoto, T. (登録日: 2007-06-25, 公開日: 2008-04-08, 最終更新日: 2024-10-16)

主引用文献

Kakuta, Y.,Okino, N.,Kajiwara, H.,Ichikawa, M.,Takakura, Y.,Ito, M.,Yamamoto, T.
Crystal structure of Vibrionaceae Photobacterium sp. JT-ISH-224 alpha2,6-sialyltransferase in a ternary complex with donor product CMP and acceptor substrate lactose: catalytic mechanism and substrate recognition
Glycobiology, 18:66-73, 2008

PubMed Abstract: Sialyltransferases are a family of glycosyltransferases that catalyze the transfer of N-acetylneuraminic acid residues from cytidine monophosphate N-acetylneuraminic acid (CMP-NeuAc) as a donor substrate to the carbohydrate groups of glycoproteins and glycolipids as acceptor substrates. We determined the crystal structure of Delta16psp26ST, the N-terminal truncated form of alpha2,6-sialyltransferase from Vibrionaceae Photobacterium sp. JT-ISH-224, complexed with a donor product CMP and an acceptor substrate lactose. Delta16psp26ST has three structural domains. Domain 1 belongs to the immunoglobulin-like beta-sandwich fold, and domains 2 and 3 form the glycosyltransferase-B structure. The CMP and lactose were bound in the deep cleft between domains 2 and 3. In the structure, only Asp232 was within hydrogen-binding distance of the acceptor O6 carbon of the galactose residue in lactose, and His405 was within hydrogen-binding distance of the phosphate oxygen of CMP. Mutation of these residues greatly decreased the activity of the enzyme. These structural and mutational results indicated that Asp232 might act as a catalytic base for deprotonation of the acceptor substrate, and His405 might act as a catalytic acid for protonation of the donor substrate. These findings are consistent with an in-line-displacement reaction mechanism in which Delta16psp26ST catalyzes the inverting transfer reaction. Unlike the case with multifunctional sialyltransferase (Delta24PmST1) complexed with CMP and lactose, the crystal structure of which was recently reported, the alpha2,6 reaction specificity of Delta16psp26ST is likely to be determined by His123.

PubMed: 17962295
DOI: 10.1093/glycob/cwm119

実験手法

X-RAY DIFFRACTION (2.5 Å)