2Z23

Crystal structure of Y.pestis oligo peptide binding protein OppA with tri-lysine ligand

2Z23 の概要

• エントリーDOI: 10.2210/pdb2z23/pdb
• 分子名称: Periplasmic oligopeptide-binding protein, peptide (LYS)(LYS)(LYS) (3 entities in total)
• 機能のキーワード: abc transporter, periplasmic binding protein, oligopeptide, peptide binding protein
• 由来する生物種: Yersinia pestis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59264.71

構造登録者

Tanabe, M.,Bertland, T.,Mirza, O.,Byrne, B.,Brown, K.A. (登録日: 2007-05-17, 公開日: 2007-10-30, 最終更新日: 2024-11-06)

主引用文献

Tanabe, M.,Mirza, O.,Bertrand, T.,Atkins, H.S.,Titball, R.W.,Iwata, S.,Brown, K.A.,Byrne, B.
Structures of OppA and PstS from Yersinia pestis indicate variability of interactions with transmembrane domains.
Acta Crystallogr.,Sect.D, 63:1185-1193, 2007

PubMed Abstract: Bacterial ATP-binding cassette (ABC) transport systems couple ATP hydrolysis with the uptake and efflux of a wide range of substances across bacterial membranes. These systems are comprised of transmembrane domains, nucleotide binding domains and, in the case of uptake systems, periplasmic binding proteins responsible for binding and presentation of substrate to the transmembrane domains. In pathogenic bacteria, ABC systems are known to play roles in virulence and pathogenicity and the surface localization of some components has made them attractive targets for both vaccine and anti-infective development. Here, the crystallization of five proteins (OppA, PstS, PiuA, YrbD and CysP) from Yersinia pestis, the causative agent of plague, are reported that diffracted to resolution limits ranging from 1.6 to 5 A. The first crystal structures of ABC system components from Y. pestis, OppA and PstS, are also reported here as complexes with their substrates. Comparisons of these two structures with known structures of related proteins suggest that these proteins possess versatility in substrate recognition and variations in protein-protein interactions with their cognate transmembrane domains.

PubMed: 18007034
DOI: 10.1107/S0907444907048299

実験手法

X-RAY DIFFRACTION (2 Å)