2Z0Q

Crystal structure of DH-PH domain of RhoGEF3(Xpln)

2Z0Q の概要

• エントリーDOI: 10.2210/pdb2z0q/pdb
• 分子名称: Rho guanine nucleotide exchange factor 3, SULFATE ION (3 entities in total)
• 機能のキーワード: dh-ph domain, guanine-nucleotide releasing factor, signaling protein, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm: Q91X46
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40734.60

構造登録者

Murayama, K.,Kato-Murayama, M.,Terada, T.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2007-05-07, 公開日: 2008-05-13, 最終更新日: 2024-11-20)

主引用文献

Murayama, K.,Kato-Murayama, M.,Akasaka, R.,Terada, T.,Yokoyama, S.,Shirouzu, M.
Structure of the Rho-specific guanine nucleotide-exchange factor Xpln
Acta Crystallogr.,Sect.F, 68:1455-1459, 2012

PubMed Abstract: Xpln is a guanine nucleotide-exchange factor (GEF) for Rho GTPases. A Dbl homology (DH) domain followed by a pleckstrin homology (PH) domain is a widely adopted GEF-domain architecture. The Xpln structure solely comprises these two domains. Xpln activates RhoA and RhoB, but not RhoC, although their GTPase sequences are highly conserved. The molecular mechanism of the selectivity of Xpln for Rho GTPases is still unclear. In this study, the crystal structure of the tandemly arranged DH-PH domains of mouse Xpln, with a single molecule in the asymmetric unit, was determined at 1.79 Å resolution by the multiwavelength anomalous dispersion method. The DH-PH domains of Xpln share high structural similarity with those from neuroepithelial cell-transforming gene 1 protein, PDZ-RhoGEF, leukaemia-associated RhoGEF and intersectins 1 and 2. The crystal structure indicated that the α4-α5 loop in the DH domain is flexible and that the DH and PH domains interact with each other intramolecularly, thus suggesting that PH-domain rearrangement occurs upon RhoA binding.

PubMed: 23192023
DOI: 10.1107/S1744309112045265

実験手法

X-RAY DIFFRACTION (1.79 Å)