2YYJ

Crystal structure of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase complexed with FAD and 4-hydroxyphenylacetate

2YYJ の概要

• エントリーDOI: 10.2210/pdb2yyj/pdb
• 関連するPDBエントリー: 2YYG 2YYI 2YYK 2YYL 2YYM
• 分子名称: 4-hydroxyphenylacetate-3-hydroxylase, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: structurome, riken spring-8 center, oxygnase component, 4-hydroxyphenylacetate 3-monooxygenase, two-component flavin diffusible monooxygenase, fad and a substrate complex, oxidoreductase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56035.26

構造登録者

Kim, S.-H.,Hisano, T.,Takeda, K.,Iwasaki, W.,Ebihara, A.,Miki, K. (登録日: 2007-04-30, 公開日: 2007-09-04, 最終更新日: 2023-10-25)

主引用文献

Kim, S.-H.,Hisano, T.,Takeda, K.,Iwasaki, W.,Ebihara, A.,Miki, K.
Crystal Structure of the Oxygenase Component (HpaB) of the 4-Hydroxyphenylacetate 3-Monooxygenase from Thermus thermophilus HB8
J.Biol.Chem., 282:33107-33117, 2007

PubMed Abstract: The 4-hydroxyphenylacetate (4HPA) 3-monooxygenase is involved in the initial step of the 4HPA degradation pathway and catalyzes 4HPA hydroxylation to 3,4-dihydroxyphenylacetate. This enzyme consists of two components, an oxygenase (HpaB) and a reductase (HpaC). To understand the structural basis of the catalytic mechanism of HpaB, crystal structures of HpaB from Thermus thermophilus HB8 were determined in three states: a ligand-free form, a binary complex with FAD, and a ternary complex with FAD and 4HPA. Structural analysis revealed that the binding and dissociation of flavin are accompanied by conformational changes of the loop between beta5 and beta6 and of the loop between beta8 and beta9, leading to preformation of part of the substrate-binding site (Ser-197 and Thr-198). The latter loop further changes its conformation upon binding of 4HPA and obstructs the active site from the bulk solvent. Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate.

PubMed: 17804419
DOI: 10.1074/jbc.M703440200

実験手法

X-RAY DIFFRACTION (1.66 Å)