2YVE

Crystal structure of the methylene blue-bound form of the multi-drug binding transcriptional repressor CgmR

2YVE の概要

• エントリーDOI: 10.2210/pdb2yve/pdb
• 関連するPDBエントリー: 2YVH 2zoy 2zoz
• 分子名称: Transcriptional regulator, CHLORIDE ION, 3,7-BIS(DIMETHYLAMINO)PHENOTHIAZIN-5-IUM, ... (5 entities in total)
• 機能のキーワード: transcriptional regulator, helix-turn-helix, tetr-family, transcription
• 由来する生物種: Corynebacterium glutamicum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43869.07

構造登録者

Itou, H.,Shirakihara, Y.,Tanaka, I. (登録日: 2007-04-12, 公開日: 2008-04-15, 最終更新日: 2023-10-25)

主引用文献

Itou, H.,Watanabe, N.,Yao, M.,Shirakihara, Y.,Tanaka, I.
Crystal Structures of the Multidrug Binding Repressor Corynebacteriumglutamicum CgmR in Complex with Inducers and with an Operator
J.Mol.Biol., 403:174-184, 2010

PubMed Abstract: CgmR (CGL2612) from Corynebacterium glutamicum is a multidrug-resistance-related transcription factor belonging to the TetR family, which is a protein family of widespread bacterial transcription factors typically involved in environmental response. Here, we report the crystal structures of CgmR homodimeric repressor in complex with two distinct inducers (1.95 and 1.4 Å resolution) and with an operator (2.5 Å resolution). The CgmR-operator complex showed that two CgmR dimers bound to the operator, and each half-site of the palindromic operator was asymmetrically recognized by two DNA-binding domains from different dimers on the opposite sides of the DNA. The inducer complexes demonstrated that both bound inducers act as a wedge to alter the operator-binding conformation of the repressor by steric inhibition. As steric hindrance is used, various drugs should act as inducers if they have sufficient volume for the conformation change and if their bindings sufficiently reduce free energy. The comparative structural study of CgmR free protein, in complex with operator, and with inducers, implies the other mechanism that might contribute to multidrug response of the repressor.

PubMed: 20691702
DOI: 10.1016/j.jmb.2010.07.042

実験手法

X-RAY DIFFRACTION (1.4 Å)