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2YPF

Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism

2YPF の概要
エントリーDOI10.2210/pdb2ypf/pdb
分子名称AVRBS3, 5'-D(*TP*TP*TP*AP*TP*AP*TP*AP*AP*AP*CP*CP*TP*AP *AP*CP*CP*CP*TP*CP*TP*AP)-3', 5'-D(*TP*AP*GP*AP*GP*GP*GP*TP*TP*AP*GP*GP*TP*TP *TP*AP*TP*AP*TP*AP*AP)-3', ... (4 entities in total)
機能のキーワードdna binding protein-dna complex, dna binding protein/dna
由来する生物種XANTHOMONAS CAMPESTRIS
詳細
タンパク質・核酸の鎖数3
化学式量合計91839.07
構造登録者
Stella, S.,Molina, R.,Yefimenko, I.,Prieto, J.,Silva, G.H.,Bertonati, C.,Juillerat, A.,Duchateau, P.,Montoya, G. (登録日: 2012-10-30, 公開日: 2013-08-28, 最終更新日: 2023-12-20)
主引用文献Stella, S.,Molina, R.,Yefimenko, I.,Prieto, J.,Silva, G.H.,Bertonati, C.,Juillerat, A.,Duchateau, P.,Montoya, G.
Structure of the Avrbs3-DNA Complex Provides New Insights Into the Initial Thymine-Recognition Mechanism
Acta Crystallogr.,Sect.D, 69:1707-, 2013
Cited by
PubMed Abstract: Transcription activator-like effectors contain a DNA-binding domain organized in tandem repeats. The repeats include two adjacent residues known as the repeat variable di-residue, which recognize a single base pair, establishing a direct code between the dipeptides and the target DNA. This feature suggests this scaffold as an excellent candidate to generate new protein-DNA specificities for biotechnological applications. Here, the crystal structure of AvrBs3 (residues 152-895, molecular mass 82 kDa) in complex with its target DNA sequence is presented, revealing a new mode of interaction with the initial thymine of the target sequence, together with an analysis of both the binding specificity and the thermodynamic properties of AvrBs3. This study quantifies the affinity and the specificity between AvrBs3 and its target DNA. Moreover, in vitro and in vivo analyses reveal that AvrBs3 does not show a strict nucleotide-binding preference for the nucleotide at the zero position of the DNA, widening the number of possible sequences that could be targeted by this scaffold.
PubMed: 23999294
DOI: 10.1107/S0907444913016429
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.55 Å)
構造検証レポート
Validation report summary of 2ypf
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-13に公開中

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