2YPF
Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism
2YPF の概要
エントリーDOI | 10.2210/pdb2ypf/pdb |
分子名称 | AVRBS3, 5'-D(*TP*TP*TP*AP*TP*AP*TP*AP*AP*AP*CP*CP*TP*AP *AP*CP*CP*CP*TP*CP*TP*AP)-3', 5'-D(*TP*AP*GP*AP*GP*GP*GP*TP*TP*AP*GP*GP*TP*TP *TP*AP*TP*AP*TP*AP*AP)-3', ... (4 entities in total) |
機能のキーワード | dna binding protein-dna complex, dna binding protein/dna |
由来する生物種 | XANTHOMONAS CAMPESTRIS 詳細 |
タンパク質・核酸の鎖数 | 3 |
化学式量合計 | 91839.07 |
構造登録者 | Stella, S.,Molina, R.,Yefimenko, I.,Prieto, J.,Silva, G.H.,Bertonati, C.,Juillerat, A.,Duchateau, P.,Montoya, G. (登録日: 2012-10-30, 公開日: 2013-08-28, 最終更新日: 2023-12-20) |
主引用文献 | Stella, S.,Molina, R.,Yefimenko, I.,Prieto, J.,Silva, G.H.,Bertonati, C.,Juillerat, A.,Duchateau, P.,Montoya, G. Structure of the Avrbs3-DNA Complex Provides New Insights Into the Initial Thymine-Recognition Mechanism Acta Crystallogr.,Sect.D, 69:1707-, 2013 Cited by PubMed Abstract: Transcription activator-like effectors contain a DNA-binding domain organized in tandem repeats. The repeats include two adjacent residues known as the repeat variable di-residue, which recognize a single base pair, establishing a direct code between the dipeptides and the target DNA. This feature suggests this scaffold as an excellent candidate to generate new protein-DNA specificities for biotechnological applications. Here, the crystal structure of AvrBs3 (residues 152-895, molecular mass 82 kDa) in complex with its target DNA sequence is presented, revealing a new mode of interaction with the initial thymine of the target sequence, together with an analysis of both the binding specificity and the thermodynamic properties of AvrBs3. This study quantifies the affinity and the specificity between AvrBs3 and its target DNA. Moreover, in vitro and in vivo analyses reveal that AvrBs3 does not show a strict nucleotide-binding preference for the nucleotide at the zero position of the DNA, widening the number of possible sequences that could be targeted by this scaffold. PubMed: 23999294DOI: 10.1107/S0907444913016429 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.55 Å) |
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