2YNQ

Crystal Structure of Geobacillus thermodenitrificans EssB extracellular fragment

2YNQ の概要

• エントリーDOI: 10.2210/pdb2ynq/pdb
• 関連するPDBエントリー: 4ANO
• 分子名称: ESSB, MALONATE ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: membrane protein, secretion, ess, type v, secretion system
• 由来する生物種: GEOBACILLUS THERMODENITRIFICANS
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 76590.51

構造登録者

Zoltner, M.,Fyfe, P.K.,Hunter, W.N. (登録日: 2012-10-17, 公開日: 2012-12-05, 最終更新日: 2024-05-08)

主引用文献

Zoltner, M.,Norman, D.G.,Fyfe, P.K.,El Mkami, H.,Palmer, T.,Hunter, W.N.
The Architecture of Essb, an Integral Membrane Component of the Type Vii Secretion System.
Structure, 21:595-, 2013

PubMed Abstract: The membrane-bound EssB is an integral and essential component of the bacterial type VII secretion system that can contribute to pathogenicity. The architecture of Geobacillus thermodenitrificans EssB has been investigated by combining crystallographic and EPR spectroscopic methods. The protein forms a dimer that straddles the cytoplasmic membrane. A helical fold is observed for the C-terminal segment, which is positioned on the exterior of the membrane. This segment contributes most to dimer formation. The N-terminal segment displays a structure related to the pseudokinase fold and may contribute to function by recognizing substrates or secretion system partners. The remaining part of EssB may serve as an anchor point for the secretion apparatus, which is embedded in the cytoplasmic membrane with the C-terminal domain protruding out to interact with partner proteins or components of peptidoglycan.

PubMed: 23499020
DOI: 10.1016/J.STR.2013.02.007

実験手法

X-RAY DIFFRACTION (2.4 Å)