2YMA

X-ray structure of the Yos9 dimerization domain

2YMA の概要

• エントリーDOI: 10.2210/pdb2yma/pdb
• 分子名称: PROTEIN OS-9 HOMOLOG (2 entities in total)
• 機能のキーワード: carbohydrate binding protein, quality control, endoplasmic reticulum-associated protein degradation, hrd complex, misfolded proteins, endoplasmic reticulum
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side: Q99220
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36516.73

構造登録者

Hanna, J.,Schuetz, A.,Zimmermann, F.,Behlke, J.,Sommer, T.,Heinemann, U. (登録日: 2011-06-07, 公開日: 2012-01-25, 最終更新日: 2024-05-01)

主引用文献

Hanna, J.,Schutz, A.,Zimmermann, F.,Behlke, J.,Sommer, T.,Heinemann, U.
Structural and Biochemical Basis of Yos9 Protein Dimerization and Possible Contribution to Self-Association of 3-Hydroxy-3-Methylglutaryl-Coenzyme a Reductase Degradation Ubiquitin-Ligase Complex.
J.Biol.Chem., 287:8633-, 2012

PubMed Abstract: In yeast, the membrane-bound HMG-CoA reductase degradation (HRD) ubiquitin-ligase complex is a key player of the ER-associated protein degradation pathway that targets misfolded proteins for proteolysis. Yos9, a component of the luminal submodule of the ligase, scans proteins for specific oligosaccharide modifications, which constitute a critical determinant of the degradation signal. Here, we report the crystal structure of the Yos9 domain that was previously suggested to confer binding to Hrd3, another component of the HRD complex. We observe an αβ-roll domain architecture and a dimeric assembly which are confirmed by analytical ultracentrifugation of both the crystallized domain and full-length Yos9. Our binding studies indicate that, instead of this domain, the N-terminal part of Yos9 including the mannose 6-phosphate receptor homology domain mediates the association with Hrd3 in vitro. Our results support the model of a dimeric state of the HRD complex and provide first-time evidence of self-association on its luminal side.

PubMed: 22262864
DOI: 10.1074/JBC.M111.317644

実験手法

X-RAY DIFFRACTION (2.545 Å)