2YKS

PENTAMERIC LIGAND GATED ION CHANNEL ELIC MUTANT F246A

2YKS の概要

• エントリーDOI: 10.2210/pdb2yks/pdb
• 分子名称: CYS-LOOP LIGAND-GATED ION CHANNEL (1 entity in total)
• 機能のキーワード: transport protein, membrane protein, prokaryotic cys-loop receptor, cation selective channel
• 由来する生物種: ERWINIA CHRYSANTHEMI
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (Probable): P0C7B7
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 367458.55

構造登録者

Zimmermann, I.,Dutzler, R. (登録日: 2011-05-30, 公開日: 2011-07-06, 最終更新日: 2023-12-20)

主引用文献

Zimmermann, I.,Dutzler, R.
Ligand Activation of the Prokaryotic Pentameric Ligand-Gated Ion Channel Elic.
Plos Biol., 9:1101-, 2011

PubMed Abstract: While the pentameric ligand-gated ion channel ELIC has recently provided first insight into the architecture of the family at high resolution, its detailed investigation was so far prevented by the fact that activating ligands were unknown. Here we describe a study on the functional characterization of ELIC by electrophysiology and X-ray crystallography. ELIC is activated by a class of primary amines that include the neurotransmitter GABA at high micro- to millimolar concentrations. The ligands bind to a conserved site and evoke currents that slowly desensitize over time. The protein forms cation selective channels with properties that resemble the nicotinic acetylcholine receptor. The high single channel conductance and the comparably simple functional behavior make ELIC an attractive model system to study general mechanisms of ion conduction and gating in this important family of neurotransmitter receptors.

PubMed: 21713033
DOI: 10.1371/JOURNAL.PBIO.1001101

実験手法

X-RAY DIFFRACTION (3.3 Å)