2YKD

Structure of the matrix protein from human respiratory syncytial virus

2YKD の概要

• エントリーDOI: 10.2210/pdb2ykd/pdb
• 関連するPDBエントリー: 2VQP
• 分子名称: MATRIX PROTEIN, ACETATE ION (3 entities in total)
• 機能のキーワード: viral protein, viral matrix protein, peripheral membrane protein, virion
• 由来する生物種: HUMAN RESPIRATORY SYNCYTIAL VIRUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28979.58

構造登録者

McPhee, H.K.,Carlisle, J.L.,Beeby, A.,Money, V.A.,Watson, S.M.D.,Yeo, R.P.,Sanderson, J.M. (登録日: 2011-05-26, 公開日: 2011-06-08, 最終更新日: 2023-12-20)

主引用文献

Mcphee, H.K.,Carlisle, J.L.,Beeby, A.,Money, V.A.,Watson, S.M.D.,Yeo, R.P.,Sanderson, J.M.
Influence of Lipids on the Interfacial Disposition of Respiratory Syncytical Virus Matrix Protein.
Langmuir, 27:304-, 2011

PubMed Abstract: The propensity of a matrix protein from an enveloped virus of the Mononegavirales family to associate with lipids representative of the viral envelope has been determined using label-free methods, including tensiometry and Brewster angle microscopy on lipid films at the air-water interface and atomic force microscopy on monolayers transferred to OTS-treated silicon wafers. This has enabled factors that influence the disposition of the protein with respect to the lipid interface to be characterized. In the absence of sphingomyelin, respiratory syncytial virus matrix protein penetrates monolayers composed of mixtures of phosphocholines with phosphoethanolamines or cholesterol at the air-water interface. In ternary mixtures composed of sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol, the protein exhibits two separate behaviors: (1) peripheral association with the surface of sphingomyelin-rich domains and (2) penetration of sphingomyelin-poor domains. Prolonged incubation of the protein with mixtures of phosphocholines and phosphoethanolamines leads to the formation of helical protein assemblies of uniform diameter that demonstrate an inherent propensity of the protein to assemble into a filamentous form.

PubMed: 21141948
DOI: 10.1021/LA104041N

実験手法

X-RAY DIFFRACTION (1.86 Å)