2YFP

STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP

2YFP の概要

• エントリーDOI: 10.2210/pdb2yfp/pdb
• 分子名称: PROTEIN (GREEN FLUORESCENT PROTEIN) (2 entities in total)
• 機能のキーワード: green fluorescent protein, yellow-emission variant, bioluminescence, photoactive protein, fluorescent tag, photosynthesis
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26880.33

構造登録者

Wachter, R.M.,Elsliger, M.A.,Kallio, K.,Hanson, G.T.,Remington, S.J. (登録日: 1998-08-17, 公開日: 1999-01-13, 最終更新日: 2023-11-15)

主引用文献

Wachter, R.M.,Elsliger, M.A.,Kallio, K.,Hanson, G.T.,Remington, S.J.
Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein.
Structure, 6:1267-1277, 1998

PubMed Abstract: Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T.

PubMed: 9782051
DOI: 10.1016/S0969-2126(98)00127-0

実験手法

X-RAY DIFFRACTION (2.6 Å)