2Y9K

Three-dimensional model of Salmonella's needle complex at subnanometer resolution

2Y9K の概要

• エントリーDOI: 10.2210/pdb2y9k/pdb
• 関連するPDBエントリー: 2Y9J
• EMDBエントリー: 1871 1874 1875
• 分子名称: PROTEIN INVG (1 entity in total)
• 機能のキーワード: protein transport, type iii secretion system, outer membrane ring, secretin family, c15 fold
• 由来する生物種: SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 234253.73

構造登録者

Schraidt, O.,Marlovits, T.C. (登録日: 2011-02-15, 公開日: 2011-03-23, 最終更新日: 2024-05-08)

主引用文献

Schraidt, O.,Marlovits, T.C.
Three-Dimensional Model of Salmonella'S Needle Complex at Subnanometer Resolution.
Science, 331:1192-, 2011

PubMed Abstract: Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core structure, the needle complex (NC), is a ~3.5 megadalton-sized, oligomeric, membrane-embedded complex. Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella typhimurium revealed a 24-fold symmetry for the inner rings and a 15-fold symmetry for the outer rings, giving an overall C3 symmetry. Local refinement and averaging showed the organization of the central core and allowed us to reconstruct a subnanometer composite structure of the NC, which together with confident docking of atomic structures reveal insights into its overall organization and structural requirements during assembly.

PubMed: 21385715
DOI: 10.1126/SCIENCE.1199358

実験手法

ELECTRON MICROSCOPY (8.3 Å)