2Y9J

THREE-DIMENSIONAL MODEL OF SALMONELLA'S NEEDLE COMPLEX AT SUBNANOMETER RESOLUTION

Summary for 2Y9J

• Entry DOI: 10.2210/pdb2y9j/pdb
• Related: 2Y9K
• EMDB information: 1871 1874 1875
• Descriptor: PROTEIN PRGH, LIPOPROTEIN PRGK (3 entities in total)
• Functional Keywords: protein transport, type iii secretion, ir1, inner membrane ring, c24-fold
• Biological source: SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; More
• Total number of polymer chains: 48
• Total formula weight: 980528.09

Authors

Schraidt, O.,Marlovits, T.C. (deposition date: 2011-02-15, release date: 2011-03-23, Last modification date: 2024-05-08)

Primary citation

Schraidt, O.,Marlovits, T.C.
Three-Dimensional Model of Salmonella'S Needle Complex at Subnanometer Resolution.
Science, 331:1192-1195, 2011

PubMed Abstract: Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core structure, the needle complex (NC), is a ~3.5 megadalton-sized, oligomeric, membrane-embedded complex. Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella typhimurium revealed a 24-fold symmetry for the inner rings and a 15-fold symmetry for the outer rings, giving an overall C3 symmetry. Local refinement and averaging showed the organization of the central core and allowed us to reconstruct a subnanometer composite structure of the NC, which together with confident docking of atomic structures reveal insights into its overall organization and structural requirements during assembly.

PubMed: 21385715
DOI: 10.1126/science.1199358

Experimental method

ELECTRON MICROSCOPY (6.4 Å)