2Y8R

Crystal structure of apo AMA1 mutant (Tyr230Ala) from Toxoplasma gondii

2Y8R の概要

• エントリーDOI: 10.2210/pdb2y8r/pdb
• 関連するPDBエントリー: 2Y8S 2Y8T
• 分子名称: APICAL MEMBRANE ANTIGEN, PUTATIVE, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: membrane protein, moving junction, invasion
• 由来する生物種: TOXOPLASMA GONDII
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 192570.28

構造登録者

Tonkin, M.L.,Roques, M.,Lamarque, M.H.,Pugniere, M.,Douguet, D.,Crawford, J.,Lebrun, M.,Boulanger, M.J. (登録日: 2011-02-10, 公開日: 2011-08-03, 最終更新日: 2024-11-13)

主引用文献

Tonkin, M.L.,Roques, M.,Lamarque, M.H.,Pugniere, M.,Douguet, D.,Crawford, J.,Lebrun, M.,Boulanger, M.J.
Host Cell Invasion by Apicomplexan Parasites: Insights from the Co-Structure of Ama1 with a Ron2 Peptide
Science, 333:463-, 2011

PubMed Abstract: Apicomplexan parasites such as Toxoplasma gondii and Plasmodium species actively invade host cells through a moving junction (MJ) complex assembled at the parasite-host cell interface. MJ assembly is initiated by injection of parasite rhoptry neck proteins (RONs) into the host cell, where RON2 spans the membrane and functions as a receptor for apical membrane antigen 1 (AMA1) on the parasite. We have determined the structure of TgAMA1 complexed with a RON2 peptide at 1.95 angstrom resolution. A stepwise assembly mechanism results in an extensive buried surface area, enabling the MJ complex to resist the mechanical forces encountered during host cell invasion. Besides providing insights into host cell invasion by apicomplexan parasites, the structure offers a basis for designing therapeutics targeting these global pathogens.

PubMed: 21778402
DOI: 10.1126/SCIENCE.1204988

実験手法

X-RAY DIFFRACTION (2.45 Å)