2Y4Q
Solution structure of the EF-hand domain of Human Polycystin 2
Summary for 2Y4Q
| Entry DOI | 10.2210/pdb2y4q/pdb |
| NMR Information | BMRB: 17621 |
| Descriptor | POLYCYSTIN-2, CALCIUM ION (2 entities in total) |
| Functional Keywords | transport protein |
| Biological source | HOMO SAPIENS (HUMAN) |
| Cellular location | Cell projection, cilium membrane ; Multi-pass membrane protein : Q13563 |
| Total number of polymer chains | 1 |
| Total formula weight | 9099.78 |
| Authors | Allen, M.D.,Qamar, S.,Sandford, R.N. (deposition date: 2011-01-07, release date: 2011-12-21, Last modification date: 2024-05-15) |
| Primary citation | Allen, M.D.,Qamar, S.,Vadivelu, M.K.,Sandford, R.N.,Bycroft, M. A High-Resolution Structure of the EF-Hand Domain of Human Polycystin-2. Protein Sci., 23:1301-, 2014 Cited by PubMed Abstract: Autosomal dominant polycystic kidney disease (ADPKD) affects over 1:1000 of the worldwide population and is caused by mutations in two genes, PKD1 and PKD2. PKD2 encodes a 968-amino acid membrane spanning protein, Polycystin-2 (PC-2), which is a member of the TRP ion channel family. The C-terminal cytoplasmic tail contains an EF-hand motif followed by a short coiled-coil domain. We have determined the structure of the EF-hand region of PC-2 using NMR spectroscopy. The use of different boundaries, compared with those used in previous studies, have enabled us to determine a high resolution structure and show that the EF hand motif forms a standard calcium-binding pocket. The affinity of this pocket for calcium has been measured and mutants that both decrease and increase its affinity for the metal ion have been created. PubMed: 24990821DOI: 10.1002/PRO.2513 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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