2Y3C

Treponema denticola variable protein 1

2Y3C の概要

• エントリーDOI: 10.2210/pdb2y3c/pdb
• 分子名称: TREPONEMA DENTICOLA VARIABLE PROTEIN 1, ACETATE ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: unknown function, periodontal disease
• 由来する生物種: TREPONEMA DENTICOLA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32747.81

構造登録者

Le Coq, J.,Ghosh, P. (登録日: 2010-12-20, 公開日: 2011-08-31, 最終更新日: 2011-11-02)

主引用文献

Le Coq, J.,Ghosh, P.
Conservation of the C-Type Lectin Fold for Massive Sequence Variation in a Treponema Diversity-Generating Retroelement.
Proc.Natl.Acad.Sci.USA, 108:14649-, 2011

PubMed Abstract: Anticipatory ligand binding through massive protein sequence variation is rare in biological systems, having been observed only in the vertebrate adaptive immune response and in a phage diversity-generating retroelement (DGR). Earlier work has demonstrated that the prototypical DGR variable protein, major tropism determinant (Mtd), meets the demands of anticipatory ligand binding by novel means through the C-type lectin (CLec) fold. However, because of the low sequence identity among DGR variable proteins, it has remained unclear whether the CLec fold is a general solution for DGRs. We have addressed this problem by determining the structure of a second DGR variable protein, TvpA, from the pathogenic oral spirochete Treponema denticola. Despite its weak sequence identity to Mtd (∼16%), TvpA was found to also have a CLec fold, with predicted variable residues exposed in a ligand-binding site. However, this site in TvpA was markedly more variable than the one in Mtd, reflecting the unprecedented approximate 10(20) potential variability of TvpA. In addition, similarity between TvpA and Mtd with formylglycine-generating enzymes was detected. These results provide strong evidence for the conservation of the formylglycine-generating enzyme-type CLec fold among DGRs as a means of accommodating massive sequence variation.

PubMed: 21873231
DOI: 10.1073/PNAS.1105613108

実験手法

X-RAY DIFFRACTION (1.4 Å)