2XZK

THE ASPERGILLUS FUMIGATUS SIALIDASE IS A KDNASE: STRUCTURAL AND MECHANISTIC INSIGHTS

2XZK の概要

• エントリーDOI: 10.2210/pdb2xzk/pdb
• 関連するPDBエントリー: 2XCY 2XZI 2XZJ
• 分子名称: EXTRACELLULAR SIALIDASE/NEURAMINIDASE, PUTATIVE, 3-deoxy-3-fluoro-D-erythro-alpha-L-manno-non-2-ulopyranosonic acid, (2R,3R,4R,5R,6S)-2,3-bis(fluoranyl)-4,5-bis(oxidanyl)-6-[(1R,2R)-1,2,3-tris(oxidanyl)propyl]oxane-2-carboxylic acid, ... (8 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: ASPERGILLUS FUMIGATUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86888.18

構造登録者

Telford, J.C.,Yeung, J.H.F.,Kiefel, M.J.,Watts, A.G.,Hader, S.,Chan, J.,Bennet, A.J.,Moore, M.M.,Taylor, G.L. (登録日: 2010-11-26, 公開日: 2011-01-19, 最終更新日: 2024-11-13)

主引用文献

Telford, J.C.,Yeung, J.H.F.,Xu, G.,Kiefel, M.J.,Watts, A.G.,Hader, S.,Chan, J.,Bennet, A.J.,Moore, M.M.,Taylor, G.L.
The Aspergillus Fumigatus Sialidase is a Kdnase: Structural and Mechanistic Insights.
J.Biol.Chem., 286:10783-, 2011

PubMed Abstract: Aspergillus fumigatus is a filamentous fungus that can cause severe respiratory disease in immunocompromised individuals. A putative sialidase from A. fumigatus was recently cloned and shown to be relatively poor in cleaving N-acetylneuraminic acid (Neu5Ac) in comparison with bacterial sialidases. Here we present the first crystal structure of a fungal sialidase. When the apo structure was compared with bacterial sialidase structures, the active site of the Aspergillus enzyme suggested that Neu5Ac would be a poor substrate because of a smaller pocket that normally accommodates the acetamido group of Neu5Ac in sialidases. A sialic acid with a hydroxyl in place of an acetamido group is 2-keto-3-deoxynononic acid (KDN). We show that KDN is the preferred substrate for the A. fumigatus sialidase and that A. fumigatus can utilize KDN as a sole carbon source. A 1.45-Å resolution crystal structure of the enzyme in complex with KDN reveals KDN in the active site in a boat conformation and nearby a second binding site occupied by KDN in a chair conformation, suggesting that polyKDN may be a natural substrate. The enzyme is not inhibited by the sialidase transition state analog 2-deoxy-2,3-dehydro-N-acetylneuraminic acid (Neu5Ac2en) but is inhibited by the related 2,3-didehydro-2,3-dideoxy-D-glycero-D-galacto-nonulosonic acid that we show bound to the enzyme in a 1.84-Å resolution crystal structure. Using a fluorinated KDN substrate, we present a 1.5-Å resolution structure of a covalently bound catalytic intermediate. The A. fumigatus sialidase is therefore a KDNase with a similar catalytic mechanism to Neu5Ac exosialidases, and this study represents the first structure of a KDNase.

PubMed: 21247893
DOI: 10.1074/JBC.M110.207043

実験手法

X-RAY DIFFRACTION (1.5 Å)