2XZ4

Crystal structure of the LFZ ectodomain of the peptidoglycan recognition protein LF

2XZ4 の概要

• エントリーDOI: 10.2210/pdb2xz4/pdb
• 関連するPDBエントリー: 2XZ8
• 分子名称: PEPTIDOGLYCAN-RECOGNITION PROTEIN LF, COPPER (II) ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: immune system, innate immunity
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• 細胞内の位置: Membrane; Multi-pass membrane protein (Potential): Q8SXQ7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41698.82

構造登録者

Basbous, N.,Coste, F.,Leone, P.,Vincentelli, R.,Royet, J.,Kellenberger, C.,Roussel, A. (登録日: 2010-11-23, 公開日: 2011-04-13, 最終更新日: 2024-11-13)

主引用文献

Basbous, N.,Coste, F.,Leone, P.,Vincentelli, R.,Royet, J.,Kellenberger, C.,Roussel, A.
The Drosophila Peptidoglycan-Recognition Protein Lf Interacts with Peptidoglycan-Recognition Protein Lc to Downregulate the Imd Pathway.
Embo Rep., 12:327-, 2011

PubMed Abstract: The peptidoglycan (PGN)-recognition protein LF (PGRP-LF) is a specific negative regulator of the immune deficiency (Imd) pathway in Drosophila. We determine the crystal structure of the two PGRP domains constituting the ectodomain of PGRP-LF at 1.72 and 1.94 Å resolution. The structures show that the LFz and LFw domains do not have a PGN-docking groove that is found in other PGRP domains, and they cannot directly interact with PGN, as confirmed by biochemical-binding assays. By using surface plasmon resonance analysis, we show that the PGRP-LF ectodomain interacts with the PGRP-LCx ectodomain in the absence and presence of tracheal cytotoxin. Our results suggest a mechanism for downregulation of the Imd pathway on the basis of the competition between PRGP-LCa and PGRP-LF to bind to PGRP-LCx.

PubMed: 21372849
DOI: 10.1038/EMBOR.2011.19

実験手法

X-RAY DIFFRACTION (1.72 Å)