2XYZ

De Novo model of Bacteriophage P22 virion coat protein

Summary for 2XYZ

• Entry DOI: 10.2210/pdb2xyz/pdb
• Related: 2XYY
• EMDB information: 1824 1826
• Descriptor: COAT PROTEIN (1 entity in total)
• Functional Keywords: virus, maturation, dsdna virus
• Biological source: ENTEROBACTERIA PHAGE P22
• Total number of polymer chains: 7
• Total formula weight: 327569.29

Authors

Chen, D.-H.,Baker, M.L.,Hryc, C.F.,DiMaio, F.,Jakana, J.,Wu, W.,Dougherty, M.,Haase-Pettingell, C.,Schmid, M.F.,Jiang, W.,Baker, D.,King, J.A.,Chiu, W. (deposition date: 2010-11-19, release date: 2011-02-02, Last modification date: 2024-05-08)

Primary citation

Chen, D.-H.,Baker, M.L.,Hryc, C.F.,Dimaio, F.,Jakana, J.,Wu, W.,Dougherty, M.,Haase-Pettingell, C.,Schmid, M.F.,Jiang, W.,Baker, D.,King, J.A.,Chiu, W.
Structural Basis for Scaffolding-Mediated Assembly and Maturation of a DsDNA Virus.
Proc.Natl.Acad.Sci.USA, 108:1355-, 2011

PubMed Abstract: Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This process, conserved among dsDNA viruses such as herpes viruses and bacteriophages, is key to forming infectious virions. Bacteriophage P22 has served as a model system for this study in the past several decades. However, how capsid assembly is initiated, where and how scaffolding proteins bind to coat proteins in the procapsid, and the conformational changes upon capsid maturation still remain elusive. Here, we report Cα backbone models for the P22 procapsid and infectious virion derived from electron cryomicroscopy density maps determined at 3.8- and 4.0-Å resolution, respectively, and the first procapsid structure at subnanometer resolution without imposing symmetry. The procapsid structures show the scaffolding protein interacting electrostatically with the N terminus (N arm) of the coat protein through its C-terminal helix-loop-helix motif, as well as unexpected interactions between 10 scaffolding proteins and the 12-fold portal located at a unique vertex. These suggest a critical role for the scaffolding proteins both in initiating the capsid assembly at the portal vertex and propagating its growth on a T = 7 icosahedral lattice. Comparison of the procapsid and the virion backbone models reveals coordinated and complex conformational changes. These structural observations allow us to propose a more detailed molecular mechanism for the scaffolding-mediated capsid assembly initiation including portal incorporation, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.

PubMed: 21220301
DOI: 10.1073/PNAS.1015739108

Experimental method

ELECTRON MICROSCOPY (4 Å)