2XSL

The crystal structure of a Thermus thermophilus tRNAGly acceptor stem microhelix at 1.6 Angstroem resolution

Summary for 2XSL

• Entry DOI: 10.2210/pdb2xsl/pdb
• Descriptor: 5'-R(*GP*CP*GP*GP*GP*AP*G)-3', 5'-R(*CP*UP*CP*CP*CP*GP*C)-3' (3 entities in total)
• Functional Keywords: rna, identity elements, glycyl-trna synthetase (glyrs), rna hydration
• Biological source: THERMUS THERMOPHILUS; More
• Total number of polymer chains: 4
• Total formula weight: 8895.56

Authors

Oberthuer, D.,Eichert, A.,Erdmann, V.A.,Fuerste, J.P.,Betzel, C.,Foerster, C. (deposition date: 2010-10-29, release date: 2011-08-10, Last modification date: 2024-05-01)

Primary citation

Oberthur, D.,Eichert, A.,Erdmann, V.A.,Furste, J.P.,Betzel, C.,Forster, C.
The Crystal Structure of a Thermus Thermophilus tRNA(Gly) Acceptor Stem Microhelix at 1.6 A Resolution.
Biochem.Biophys.Res.Commun., 404:245-, 2011

PubMed Abstract: tRNAs are aminoacylated with the correct amino acid by the cognate aminoacyl-tRNA synthetase. The tRNA/synthetase systems can be divided into two classes: class I and class II. Within class I, the tRNA identity elements that enable the specificity consist of complex sequence and structure motifs, whereas in class II the identity elements are assured by few and simple determinants, which are mostly located in the tRNA acceptor stem. The tRNA(Gly)/glycyl-tRNA-synthetase (GlyRS) system is a special case regarding evolutionary aspects. There exist two different types of GlyRS, namely an archaebacterial/human type and an eubacterial type, reflecting the evolutionary divergence within this system. We previously reported the crystal structures of an Escherichia coli and of a human tRNA(Gly) acceptor stem microhelix. Here we present the crystal structure of a thermophilic tRNA(Gly) aminoacyl stem from Thermus thermophilus at 1.6Å resolution and provide insight into the RNA geometry and hydration.

PubMed: 21114959
DOI: 10.1016/J.BBRC.2010.11.101

Experimental method

X-RAY DIFFRACTION (1.59 Å)