2XRO

Crystal structure of TtgV in complex with its DNA operator

2XRO の概要

• エントリーDOI: 10.2210/pdb2xro/pdb
• 関連するPDBエントリー: 2XRN
• 分子名称: HTH-TYPE TRANSCRIPTIONAL REGULATOR TTGV, TTGV OPERATOR DNA, OSMIUM ION, ... (4 entities in total)
• 機能のキーワード: dna-binding protein-dna complex, tetrameric gene regulator, cooperative dna binding, multidrug binding protein, antibiotic resistance, complex (dna-binding protein-dna ), dna-binding protein/dna
• 由来する生物種: PSEUDOMONAS PUTIDA; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 128969.58

構造登録者

Lu, D.,Fillet, S.,Meng, C.,Alguel, Y.,Kloppsteck, P.,Bergeron, J.,Krell, T.,Gallegos, M.-T.,Ramos, J.,Zhang, X. (登録日: 2010-09-17, 公開日: 2010-12-01, 最終更新日: 2023-12-20)

主引用文献

Lu, D.,Fillet, S.,Meng, C.,Alguel, Y.,Kloppsteck, P.,Bergeron, J.,Krell, T.,Gallegos, M.T.,Ramos, J.,Zhang, X.
Crystal Structure of Ttgv in Complex with its DNA Operator Reveals a General Model for Cooperative DNA Binding of Tetrameric Gene Regulators.
Genes Dev., 24:2556-, 2010

PubMed Abstract: The majority of bacterial gene regulators bind as symmetric dimers to palindromic DNA operators of 12-20 base pairs (bp). Multimeric forms of proteins, including tetramers, are able to recognize longer operator sequences in a cooperative manner, although how this is achieved is not well understood due to the lack of complete structural information. Models, instead of structures, of complete tetrameric assembly on DNA exist in literature. Here we present the crystal structures of the multidrug-binding protein TtgV, a gene repressor that controls efflux pumps, alone and in complex with a 42-bp DNA operator containing two TtgV recognition sites at 2.9 Å and 3.4 Å resolution. These structures represent the first full-length functional tetrameric protein in complex with its intact DNA operator containing two continuous recognition sites. TtgV binds to its DNA operator as a highly asymmetric tetramer and induces considerable distortions in the DNA, resulting in a 60° bend. Upon binding to its operator, TtgV undergoes large conformational changes at the monomeric, dimeric, and tetrameric levels. The structures here reveal a general model for cooperative DNA binding of tetrameric gene regulators and provide a structural basis for a large body of biochemical data and a reinterpretation of previous models for tetrameric gene regulators derived from partial structural data.

PubMed: 21078819
DOI: 10.1101/GAD.603510

実験手法

X-RAY DIFFRACTION (3.4 Å)