2XQX

Structure of the family 32 carbohydrate-binding module from Streptococcus pneumoniae EndoD

2XQX の概要

• エントリーDOI: 10.2210/pdb2xqx/pdb
• 関連するPDBエントリー: 2W91 2W92
• 分子名称: ENDO-BETA-N-ACETYLGLUCOSAMINIDASE D, CALCIUM ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: sugar binding protein, carbohydrate binding, beta-sandwhich
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (By similarity): Q93HW0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34118.66

構造登録者

Abbott, D.W.,Boraston, A.B. (登録日: 2010-09-07, 公開日: 2010-11-03, 最終更新日: 2024-10-23)

主引用文献

Abbott, D.W.,Boraston, A.B.
Structural Analysis of a Putative Family 32 Carbohydrate-Binding Module from the Streptococcus Pneumoniae Enzyme Endod.
Acta Crystallogr.,Sect.F, 67:429-, 2011

PubMed Abstract: EndoD is an architecturally complex endo-β-1,4-N-acetylglucosamidase from Streptococcus pneumoniae that cleaves the chitobiose core of N-linked glycans and contributes to pneumococcal virulence. Although the glycoside hydrolase family 85 catalytic module has been structurally and functionally characterized, nothing is known about the ancillary modules and how they contribute to the overall function of the enzyme. Presented here is the 2.0 Å resolution structure of a family 32 carbohydrate-binding module of EndoD, SpCBM32, solved by single-wavelength anomalous dispersion. The putative binding site of this protein is a charge-neutral relatively flat region on the protein surface that contains one prominently exposed tryptophan residue that extends into the solvent. These topographical features are discussed in the biological context of EndoD activity and a hypothesis is made about the complex structure of its potential carbohydrate ligand.

PubMed: 21505233
DOI: 10.1107/S1744309111001874

実験手法

X-RAY DIFFRACTION (2.01 Å)