2XOD

Crystal structure of flavoprotein NrdI from Bacillus anthracis in the oxidised form

2XOD の概要

• エントリーDOI: 10.2210/pdb2xod/pdb
• 関連するPDBエントリー: 2X2O 2X2P 2XOE
• 分子名称: NRDI PROTEIN, FLAVIN MONONUCLEOTIDE, ZINC ION, ... (5 entities in total)
• 機能のキーワード: flavoprotein, redox protein, ribonucleotide reductase
• 由来する生物種: Bacillus anthracis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14123.97

構造登録者

Johansson, R.,Sprenger, J.,Torrents, E.,Sahlin, M.,Sjoberg, B.M.,Logan, D.T. (登録日: 2010-08-14, 公開日: 2010-08-25, 最終更新日: 2023-12-20)

主引用文献

Johansson, R.,Torrents, E.,Lundin, D.,Sprenger, J.,Sahlin, M.,Sjoberg, B.M.,Logan, D.T.
High Resolution Crystal Structures of Nrdi in the Oxidised and Reduced States: An Unusual Flavodoxin
FEBS J., 277:4265-, 2010

PubMed Abstract: The small flavoprotein NrdI is an essential component of the class Ib ribonucleotide reductase system in many bacteria. NrdI interacts with the class Ib radical generating protein NrdF. It is suggested to be involved in the rescue of inactivated diferric centres or generation of active dimanganese centres in NrdF. Although NrdI bears a superficial resemblance to flavodoxin, its redox properties have been demonstrated to be strikingly different. In particular, NrdI is capable of two-electron reduction, whereas flavodoxins are exclusively one-electron reductants. This has been suggested to depend on a lesser destabilization of the negatively-charged hydroquinone state than in flavodoxins. We have determined the crystal structures of NrdI from Bacillus anthracis, the causative agent of anthrax, in the oxidized and semiquinone forms, at resolutions of 0.96 and 1.4 Å, respectively. These structures, coupled with analysis of all curated NrdI sequences, suggest that NrdI defines a new structural family within the flavodoxin superfamily. The conformational behaviour of NrdI in response to FMN reduction is very similar to that of flavodoxins, involving a peptide flip in a loop near the N5 atom of the flavin ring. However, NrdI is much less negatively charged than flavodoxins, which is expected to affect its redox properties significantly. Indeed, sequence analysis shows a remarkable spread in the predicted isoelectric points of NrdIs, from approximately pH 4-10. The implications of these observations for class Ib ribonucleotide reductase function are discussed.

PubMed: 20831589
DOI: 10.1111/J.1742-4658.2010.07815.X

実験手法

X-RAY DIFFRACTION (0.96 Å)