2XLI

Crystal structure of the Csy4-crRNA complex, monoclinic form

2XLI の概要

• エントリーDOI: 10.2210/pdb2xli/pdb
• 関連するPDBエントリー: 2XLJ 2XLK
• 分子名称: CSY4 ENDORIBONUCLEASE, 5'-R(*CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP*CP*A*DG*C)-3' (3 entities in total)
• 機能のキーワード: hydrolase-rna complex, endoribonuclease, crispr, hydrolase/rna
• 由来する生物種: PSEUDOMONAS AERUGINOSA; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26947.03

構造登録者

Haurwitz, R.E.,Jinek, M.,Wiedenheft, B.,Zhou, K.,Doudna, J.A. (登録日: 2010-07-20, 公開日: 2010-09-22, 最終更新日: 2024-05-08)

主引用文献

Haurwitz, R.E.,Jinek, M.,Wiedenheft, B.,Zhou, K.,Doudna, J.A.
Sequence- and Structure-Specific RNA Processing by a Crispr Endonuclease.
Science, 329:1355-, 2010

PubMed Abstract: Many bacteria and archaea contain clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) after transcription of the CRISPR locus. Here, we identify the endoribonuclease (Csy4) responsible for CRISPR transcript (pre-crRNA) processing in Pseudomonas aeruginosa. A 1.8 angstrom crystal structure of Csy4 bound to its cognate RNA reveals that Csy4 makes sequence-specific interactions in the major groove of the crRNA repeat stem-loop. Together with electrostatic contacts to the phosphate backbone, these enable Csy4 to bind selectively and cleave pre-crRNAs using phylogenetically conserved serine and histidine residues in the active site. The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases.

PubMed: 20829488
DOI: 10.1126/SCIENCE.1192272

実験手法

X-RAY DIFFRACTION (2.33 Å)