2XJS

X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and a1,2-mannobiose

Summary for 2XJS

• Entry DOI: 10.2210/pdb2xjs/pdb
• Related: 2XJP 2XJQ 2XJR 2XJT 2XJU 2XJV
• Related PRD ID: PRD_900111
• Descriptor: FLOCCULATION PROTEIN FLO5, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: cell adhesion, greenbeard, social interaction, pa14-domain, carbohydrate binding
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• Total number of polymer chains: 1
• Total formula weight: 28236.40

Authors

Veelders, M.,Brueckner, S.,Ott, D.,Unverzagt, C.,Moesch, H.-U.,Essen, L.-O. (deposition date: 2010-07-06, release date: 2010-12-15, Last modification date: 2024-05-01)

Primary citation

Veelders, M.,Brueckner, S.,Ott, D.,Unverzagt, C.,Moesch, H.-U.,Essen, L.-O.
Structural Basis of Flocculin-Mediated Social Behavior in Yeast
Proc.Natl.Acad.Sci.USA, 107:22511-, 2010

PubMed Abstract: In the budding yeast Saccharomyces cerevisiae, self-recognition and the thereby promoted aggregation of thousands of cells into protective flocs is mediated by a family of cell-surface adhesins, the flocculins (Flo). Based on this social behavior FLO genes fulfill the definition of "greenbeard" genes, which direct cooperation toward other carriers of the same gene. The process of flocculation plays an eminent role in the food industry for the production of beer and wine. However, the precise mode of flocculin-mediated surface recognition and the exact structure of cognate ligands have remained elusive. Here, we present structures of the adhesion domain of a flocculin complexed to its cognate ligands derived from yeast high-mannose oligosaccharides at resolutions up to 0.95 Å. Besides a PA14-like architecture, the Flo5A domain reveals a previously undescribed lectin fold that utilizes a unique DcisD calcium-binding motif for carbohydrate binding and that is widely spread among pro- and eukaryotes. Given the high abundance of high-mannose oligosaccharides in yeast cell walls, the Flo5A structure suggests a model for recognition, where social non-self- instead of unsocial self-interactions are favored.

PubMed: 21149680
DOI: 10.1073/PNAS.1013210108

Experimental method

X-RAY DIFFRACTION (1.3 Å)