2XG5

E. coli P pilus chaperone-subunit complex PapD-PapH bound to pilus biogenesis inhibitor, pilicide 5d

2XG5 の概要

• エントリーDOI: 10.2210/pdb2xg5/pdb
• 関連するPDBエントリー: 1N0L 1PDK 1QPP 1QPX 2J2Z 2WMP 2XG4 3DPA
• 分子名称: CHAPERONE PROTEIN PAPD, PAP FIMBRIAL MINOR PILIN PROTEIN, GLYCEROL, ... (8 entities in total)
• 機能のキーワード: chaperone, chaperone-surface active protein complex
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Periplasm: P15319; Secreted: P07111
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45039.85

構造登録者

Remaut, H.,Phan, G.,Buelens, F.,Chorell, E.,Pinkner, J.S.,Edvinsson, S.,Almqvist, F.,Hultgren, S.J.,Waksman, G. (登録日: 2010-05-30, 公開日: 2010-07-14, 最終更新日: 2024-10-09)

主引用文献

Chorell, E.,Pinkner, J.S.,Phan, G.,Edvinsson, S.,Buelens, F.,Remaut, H.,Waksman, G.,Hultgren, S.J.,Almqvist, F.
Design and Synthesis of C-2 Substituted Thiazolo and Dihydrothiazolo Ring-Fused 2-Pyridones: Pilicides with Increased Antivirulence Activity.
J.Med.Chem., 53:5690-, 2010

PubMed Abstract: Pilicides block pili formation by binding to pilus chaperones and blocking their function in the chaperone/usher pathway in E. coli. Various C-2 substituents were introduced on the pilicide scaffold by design and synthetic method developments. Experimental evaluation showed that proper substitution of this position affected the biological activity of the compound. Aryl substituents resulted in pilicides with significantly increased potencies as measured in pili-dependent biofilm and hemagglutination assays. The structural basis of the PapD chaperone-pilicide interactions was determined by X-ray crystallography.

PubMed: 20586493
DOI: 10.1021/JM100470T

実験手法

X-RAY DIFFRACTION (2 Å)