2XEC

Nocardia farcinica maleate cis-trans isomerase bound to TRIS

2XEC の概要

• エントリーDOI: 10.2210/pdb2xec/pdb
• 分子名称: PUTATIVE MALEATE ISOMERASE, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: isomerase
• 由来する生物種: NOCARDIA FARCINICA
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 115760.61

構造登録者

Fisch, F.,Martinez-Fleites, C.,Baudendistel, N.,Hauer, B.,Turkenburg, J.P.,Hart, S.,Bruce, N.C.,Grogan, G. (登録日: 2010-05-13, 公開日: 2010-08-18, 最終更新日: 2023-12-20)

主引用文献

Fisch, F.,Fleites, C.M.,Delenne, M.,Baudendistel, N.,Hauer, B.,Turkenburg, J.P.,Hart, S.,Bruce, N.C.,Grogan, G.
A Covalent Succinylcysteine-Like Intermediate in the Enzyme-Catalyzed Transformation of Maleate to Fumarate by Maleate Isomerase.
J.Am.Chem.Soc., 132:11455-, 2010

PubMed Abstract: Maleate isomerase (MI), a member of the Asp/Glu racemase superfamily, catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Mutational studies, in conjunction with the structure of the C194A mutant of Nocardia farcinica MI cocrystallized with maleate, have revealed an unprecedented mode of catalysis for the superfamily in which the isomerization reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate.

PubMed: 20677745
DOI: 10.1021/JA1053576

実験手法

X-RAY DIFFRACTION (2.2 Å)